Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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Enzymes All enzymes are globular proteins and round in shape They have the suffix "-ase" Intracellular enzymes are found inside the cell Extracellular enzymes act outside the cell (e.g. digestive enzymes) Enzymes are catalysts → speed up chemical reactions Reduce activation energy required to start a reaction between molecules Substrates (reactants) are converted into products Reaction may not take place in absence of enzymes (each enzyme has a specific catalytic action) Enzymes catalyse
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Enzyme Activity Lab Hypothesis: 1] Are enzymes reusable? Yes‚ enzymes are reusable. 2] Which of the following do you think contain catalase? [Manganese dioxide‚ chicken meat‚ apple‚ carrot‚ potato‚ or chicken liver] I think that the manganese dioxide‚ apple‚ and carrot contain the catalase. 3] Would grinding the materials affect enzyme activity? I think that grinding the materials will increase enzyme activity. 4] Would temperature affect enzyme activity? I think that
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Enzyme Report Case 1 - Hereditary Fructose Intolerance 1 & 2. Enzymes take on a variety of roles in the human body at the cellular level. Specifically‚ they aid in the breakdown of macronutrients such as glucose and fructose so that the body can use them. Although reactions within the body would occur without enzymes‚ enzymes provide control because the reaction is not needed all of the time. Enzymes function in specific environments such as temperature or pH; some enzymes are specific
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introduction to enzymes Enzymes are the foundation of energy and the life force in all living things. They are responsible for building‚ detoxifying‚ and healing the body. They are also the force that allows your body to digest and absorb food. Enzymes also regulate tens of thousands of other biochemical functions that take place in the body every day. Without enzymes‚ seeds would not sprout‚ fruit would not ripen‚ leaves would not change color‚ and life would not exist. Therefore‚ the study of enzymes has
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TASK 4: Explain the structure of the ileum and the ultrastructure of the epithelium in relation to its secretory and absorptive functions. (a) Explain the characteristics of enzymes. (b) Explain the lock and key theory of enzyme action. (c) Locate and explain the role of important digestive enzymes. TASK 5: Enzymes are involved in anabolic and catabolic reactions: a) Define the terms anabolic and catabolic reactions b) Give 3 examples of anabolic and 3 examples of catabolic reactions
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Enzymes Lecture outlines •Catalysis profile •Activation energy & its •Enzyme & substrate substrates •How enzymes bind to •Lock & Key model •Induced-fit model •Enzyme assay Lecture outcomes • At the end of this lecture‚ students are able to: • Define the catalyst • Understand how enzymes work as catalysts‚ the concept of activation energy and enzymes-substrate binding • Explain different theories of the relation between enzymes and substrates Catalysis • It is probably
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temperature was above 40Ëšc the enzyme did not work as efficiently‚ even with the extra energy‚ as they had become deformed. Where the enzyme does not work so well or does not even work at all the active site if the enzyme had changed. The enzyme had not died as it is not a living organism. With the shape of the active site changed the enzyme is unable to perform the "lock and key" action the enzyme is meant to do in order to catalyse a reaction. The specified enzyme is shaped to "lock" on to
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Enzyme Catalase What Factors Affect Enzyme Activity Michelina Bartolotto Lab Biology 111B February 2‚ 2014 /media/common.studymode/studymode-upload/stm/files/e1b9a3d6adf94ca848b12159c31f11b0.docx INTRODUCTION Enzymes are proteins that function as biological catalysts (Perry‚ Morton 2007). They maintain the body’s stable internal balance‚ and without them life would
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Discovery Restriction enzymes were discovered 40 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Restriction enzymes protect bacteria from infections by viruses‚ and it is generally accepted that this is their role in nature. They function as microbial immune systems. When a strain of E. coli lacking a restriction enzyme is infected with a virus‚ most virus particles can initiate a successful infection. When the same strain
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