Clinical Chemistry Manlangit‚ Joyce Ann U. EXPERIMENT Title: Standard Curve Preparation Objectives: 1. Properly set up a standard curve 2. Using the prepared curve‚ determine the concentrations for control and patient specimens. Materials and Reagents: 2ml 1M Sulfuric Acid 2ml of 0.1M Potassium Dichromate Distilled Water Pipettes Volumetric Flask Test Tubes Procedure: 1. Prepare 1 ml glucose solution and place in test tubes 2. Add 2ml of 1M Sulfuric Acid solution 3. Add
Premium Laboratory glassware Carbon Chemistry
bell curve is literally‚ the symmetrical curve created on a graph when using a frequency distribution method for a set of data‚ splitting the mean symmetrically. There is a big difference between standard deviation and the bell curve! Standard deviation shows the difference in variation from the average; the bell curve‚ also normal distribution or Gaussian distribution‚ shows the standard deviation and is created by the normal or equal distribution of the mean among either half. The bell curve is an
Premium Normal distribution Standard deviation
Johnson Spectrophotometer; the finding of protein concentration of an unknown sample of BSA‚ and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of the unknown solution of BSA together with
Premium Concentration Serum albumin Absorbance
whole class is stated on Figure 2. Table 3 shows the measurement on the amount of strawberry DNA extracted. In Figure 1 and Figure 2‚ the data will be presented Standard Curve Graph. The relationship between the concentration of Methylene Blue and the absorbance is expected to be a direct proportion. Figure 1: Standard Curve of light absorbance by ten different concentrations of Methylene Blue. The data of the figure is collected by our group Mitochondria. The relationship between two
Premium Line Measurement Concentration
Construction of a Standard Curve for the Determination of Protein Concentration 09/13/12 PCB 3023-007 Results Figure1: Standard curve. This graph was constructed from the fixed amounts of Bovine Serum Albumin (BSA). Varying amounts of BSA at a concentration of .5µ/µl were mixed with the water and 1mL of coomassie dye. The equation y=0.0259x +0.0511 represents the best fit line for a data set taken from µg of protein equals two to ten µg. Optical density (OD) was surveyed using a Mach
Premium Serum albumin ELISA Multiplication
macroeconomics and is used by a broad array of economists‚ from libertarian‚ monetarist supporters of laissez-faire‚ such as Milton Friedman to Post-Keynesian supporters of economic interventionism‚ such as Joan Robinson. Brief history of demand curve and supply curve According to Hamid S. Hosseini‚ the power of supply and demand was understood to some extent by several early Muslim economists‚ such as Ibn Taymiyyah who illustrates- “If desire for goods increases while its availability decreases‚ its price
Premium Supply and demand Economics
1 Practical report: Determination of concentration using a standard curve Introduction Spectrophotometer is an instrument that confirms the measurement of specified wavelength of light that passes through medium. The measurement of light absorbance by a solution is also done with spectrophometer. Absorption spectrum Spectrometers are mainly and widely functional in identifying the components of solutions that helps determine the components’ concentrations. Compounds are experimentally identified
Premium Concentration Chemistry
Enzyme Lab Name ___________________________ Assignment 1: Getting to Know Enzyme Lab: Setting Up an Experiment The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments. Click on each item in the lab to learn more about its purpose. Once you are familiar with the lab‚ click on the Experiment button to begin the first assignment. This assignment is designed to help you become
Premium PH Enzyme inhibitor Enzyme
__________________________________ AP Biology LAB 2. ENZYME CATALYSIS ESSAY 2000 The effects of pH and temperature were studied for an enzyme-catalyzed reaction. The following results were obtained. a. How do (1) temperature and (2) pH affect the activity of this enzyme? In your answer‚ include a discussion of the relationship between the structure and the function of this enzyme‚ as well as a discussion of how structure and function of enzymes are affected by temperature and pH. b. Describe
Premium Allele DNA Experiment
What is Enzyme? Enzymes are protein that acts as speed up reactions and break down molecules in our body. However‚ different enzymes only work on certain types of molecules. Enzymes can accelerate the reactions by more than one million times.(3) In our human body‚ there are a total about forty thousand types of enzymes and each catalyzes different kind of molecule.(3) The molecules that enzymes help to accelerate is called substrates‚ and when enzyme is combined together with the substrate‚ it
Premium Enzyme