Biology Enzyme Catalase Investigation Aim: The aim of this investigation is to study and observe whether or not the concentration of hydrogen peroxide (varying from 10 – 30 millilitres) affects the rate of reaction. Hypothesis: With the increase of the concentration of Hydrogen Peroxide 3% substrate‚ I prediction that the rate of pressure increase will begin to amplify. The pressure is bound to increase because the catalase quickly reacts with the hydrogen peroxide; this is why the more substrate
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experiment diluted solutions of amylase were created and then tested using a starch solution‚ I2KI for reaction times. The answer to the question was proved to be that more concentration of amylase speeds up the reaction time. Introduction The enzyme‚ amylase is found in the saliva of most animals and in humans. Amylase hydrolyzes starch‚ a plant’s reservation of carbohydrates. Amylase causes a chemical reaction in the polysaccharide starch that breaks down the glucose molecules into maltose
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two Title: Enzyme Function Purpose: To observe the role of enzymes in chemical reactions and to determine the kinds of cells that contain more of the enzyme catalase. Prior Knowledge: Enzymes are proteins that assist the chemical reactions of a cell by lowering the amount of activation energy needed to start the reactions‚ thereby enabling the cell to carry out the reactions at a faster rate; enzymes that lower the activation energy are therefore called catalysts. Moreover‚ the enzyme itself is
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bung. 7. Take measurement of gas after 2 minutes. 8. Repeat. Investigating the effect of enzyme concentration on the rate of an enzyme-catalysed reaction. James Moore Conclusion: For the conclusion we see that in fact the hypothesis is correct. When independent variable increases so does the dependent variable. As the amount of enzyme concentration increases so will the rate of the enzyme catalysed reaction. In the human body substrate is always available therefore there is only one
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Introduction: Enzymes are a substance produced by a living organism that acts as a catalyst to activate a specific reaction. The purpose of this experiment was to figure out if the temperature of the reaction would rise‚ will the absorption rise as well. Reactions use energy‚ If there is energy than heat occurs. The Hypothesis that was figured out was‚ If the temperature rises‚ then the absorption will also go up. The Independent variable that was tested was temperature. The dependent variable that
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Enzyme Controlled Reactions 1) Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases? A) The relationship between the substrate concentration and the initial reaction of an enzyme-catalyzed reaction is very productive‚ but is dramatically affected by the pH level of the given solution. The most productive pH level is
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Matthew Saldanha Bio DCP lab-Catalase experiment Aim: To investigate enzyme kinetics‚ using different concentration of the enzyme. Hypothesis: The assay system used in the lab consists of a filter paper disc coated with the enzyme and the dropped into a papercup of substrate (Hydrogen Peroxide). As the hydrogen breaks down the hydrogen peroxide into hydrogen and oxygen gas‚ the bubbles of oxygen gas collect underneath the filter and make it
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The data from the experiment demonstrates that the catalase enzyme breaks down the hydrogen peroxide due to its harmful toxicity to the liver. In section A‚ the effect surface area has on the enzyme was tested. The results have proven that as the surface area increases‚ the reaction rate of the enzyme also increases. To illustrate‚ when the liver was ground‚ the bubbles from the reaction reached a maximum height of 150mm in five seconds less than the unground liver which merely reached a maximum
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Received July 18‚ 1997X A radiochemical enzyme assay for studying cyclooxygenase (COX)-catalyzed prostaglandin biosynthesis in vitro was optimized with respect to both COX-1 and COX-2 activity. The assay can be used to assess the relative selectivity of plant-derived inhibitors on COX-1 and COX-2. Assay conditions were optimized for both enzymes with respect to concentration of cofactors (l-epinephrine‚ reduced glutathione‚ and hematin)‚ activation time (enzyme and cofactors)‚ reaction time‚ and
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-APPLICATION OF ENZYMES IN ORGANIC CHEMISTRY Why enzymes in organic synthesis? | Alternative to chemical methods: * High region- and stereoselectivity * Milder reaction conditions * Environmentally more friendly | Which enzyme(s)? | OxidoreductasesHydrolasesLyasesIsomerases | Which reaction system? | AqueousAqueous and water-miscible organic solventAqueous and water-immiscible organic solventPure organic solventOther solvents (supercritical fluids‚ ionic liquids) | Which ‘chemistry’
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