BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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Enzyme kinetics (catalase/kmno4) Enzyme catalysis Farah Mohamed galal 22-3014 t09 Introduction: E + S → ES → E + P Enzymes are proteins which act as a catalyst in biochemical reactions(affect the rate of achemical reaction). The substrate binds to the active site of the enzyme. Any deformation of the active site will affect the activity of the enzyme‚ these are some ways that enzyme action may be affected because of them: 1- Salt concentration: If it is close to zero or very high
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proteins‚ carry out nearly all of a cell’s functions. A protein is made up of at least one or more amino acid polymers. The necessity of proteins for life is unequivocal‚ not only do they help to form the structures of cells but they act as enzymes in reactions of the body1. Enzymes are biological catalysts that lower the amount of activation energy needed in carrying out biochemical reactions1. Enzymes are responsible for almost every reaction that occurs in a cell and is named according to the substrate
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the issue of not legalizing the payment of donating organs. The National Kidney Foundation does not believe that the payment for organ donating will increase donations. The foundation wants to remain committed to helping people with donations. They believe that if it is legalized then disadvantaged citizens would take advantage of this opportunity that should only be done for the great feeling of it‚ and not the money. Sally Satel believes that organ trafficking should be stopped by legalizing some
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MISEP Chemistry 512 – Jacobs Enzyme Catalyst Lab - Formal Report – August 8‚ 2007 ABSTRACT This investigation examined what would happen to the rate of an enzyme-catalyzed reaction if the concentration of substrate changed. We hypothesized that if the concentration increased‚ then the reaction rate would also increase. To test our question‚ we varied a combination of substrate and buffer‚ totaling 6mL‚ with a constant amount of 2 drops of catalyst. The enzyme catalyst‚ peroxidase‚ increased
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Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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BABS1201 Group Enzymes Project Group Protocol Including Equipment and Reagents List Lab Day: Wednesday Lab Time: 10am – 1pm Are you in Lab G20 (furthest from BSB Student Office) or Lab G21 (closest to BSB Student Office)?: Demonstrator Name: Daniel Winters Names of Group Members: Johnny Nguyen‚ Therese Pham‚ Linda Tang Name of Enzyme You are Investigating: Amylase Brief Background: Amylase is a digestive enzyme‚ produced mainly by the salivary glands and the pancreas‚ to break down
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The effect of time on enzyme reaction. Abstract: In this lab investigation we will observe how the amount of hydrogen peroxide is affected by catalase over time. The enzyme was added to 10 mL’s of hydrogen peroxide and observed over time to determine the relation between time and enzyme activity. The hypothesis stated that as time increased substrate would decrease. Therefore I predicted that at 60 seconds‚ there would be the least amount of H2O2. The enzyme activity mirrored my predictions
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Organ Systems Project (Digestive‚ Excretory‚ Circulatory‚ Reproductive‚ Nervous) There is an enormous variety of life on our planet Earth ranging from simple cell bacteria to complex multicellular animals. Animals are creatures in the kingdom Animilia‚ one of the kingdoms in Whitakers 5 kingdom system. Their bodies consist of 555tanimal eukaryotic cells. Meaning their cell or cells contain a nucleus‚ are surrounded by a cell membrane (phospholipid bilayer) and can self-reproduce in a free
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INTRODUCTION OF ENZYMES Enzymes are complex proteins that cause a specific chemical change in all parts of the body (David C. Dugdale‚ 2011). When we understand enzymes we understand cells (Marshall Brian‚ 2001). In many organisms most chemical reactions are catalyzed -when a substance speeds up the rate of a chemical reaction- by enzymes. Each enzyme controls a certain function that happens in a cell. Still each one has its own process and rate that it converts molecules. Studying enzymes shows how chemical
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