Immobilization of Enzymes And Their Applications By‚ Shudhangshu Shekhar Kundu Introduction: What an enzyme? Enzymes are protein molecules‚ which serve to accelerate the chemical reactions of living cells (often by several orders of magnitude). Without enzymes‚ most biochemical reactions would be too slow to even carry out life processes. Enzymes display
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Omar Shbeeb Toothpick Enzyme Lab 9/25/13 Introduction Enzymes are used in all metabolic reactions to control the rate of reactions and decrease the amount of energy necessary for the reaction to take place. They are responsible for the thousands of chemical interconversions that sustain life. Enzymes are referred to highly selective catalysts‚ meaning they speed up the rate of metabolic reactions. To react‚ they need to find a perfect match with a substrate. They converge at a place called an
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Broadly speaking‚ enzymes are proteins that is produced to perform as a biological catalyst in chemical reactions. Catalyst are used to increase the rate of a chemical reaction. In this study‚ we performed two different experiments that investigated the effect of varying substrate concentration‚ and the effect of temperature on the rate of Enzyme-Catalase reaction. In experiment one (i.e. the effect of varying substrate concentration on the rate of enzyme-catalase reaction) we tested the hypothesis
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temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the difference absorbance levels they produced every 20 seconds for about 2 minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we got very low numbers for the absorbance‚ which gave us a lower rate for the enzyme reaction to complete‚
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Abstract: The Enzyme Lab results where when the liver was frozen‚ its reaction was fast‚ and when it was hot‚ it was slow‚ and the liver that was at room temperature reacted slowly to medium. Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature‚ pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions‚ which would otherwise
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pH & Enzyme Action Aim: To inspect the effects of the pH on enzymes. Apparatus: 100 cm³ Beaker 3 – 5cm³ Syringes 2 Test Tube Racks with 8 Test Tubes Stop-watch Ruler Dropping bottle of detergent Marker Pen Masking Tape 400cm³ Hydrogen Peroxide 200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the
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(Click on the Save a Copy button on the panel above to save your report) Activity: Name: Instructor: Date: Enzyme Activity cheryl yelton November 30‚ 2014 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable
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Enzymes are organic catalysts; where a substance speeds up the rate of chemical reactions without changing being changed by the reactions. In lab they tested this by seeing how H2O2 and the catalysts from the banana and liver react to make H2O+O2. Depending on different conditions; like decomposition of H2O2 (surface area)‚ temperature on function‚ reusing the catalase‚ reaction rate of iced liver returned to room temperature‚ and effects of pH on enzyme activity‚ to see how much O2 was released
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Factors Affecting Enzyme Activity Abstract: In the following lab factors affecting enzyme activity‚ temperature‚ pH‚ enzyme concentration‚ substrate concentration and surface area will be tested on a beef liver enzyme to see if there will be any effect of performance. By doing 2 or more trials the results will show whether there is an effect to the enzyme from the following factors or not. Some of the factors may denature the enzyme and some will do nothing. Using a table qualitative and the
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