This experiment was performed in order to analyze the effects of pH‚ enzyme concentration‚ and temperature on the catalytic rate on enzyme ALP. The initial experiment was done to approximate the optimum pH for the activity of the enzyme. The hypothesis was that alkaline phosphatase activity will be critically affected by pH effects. The hypothesis was valid because the data shows that the pH values for the acidic‚ basic‚ and neutral tubes increased over time‚ absorbing at similar rates. The basic
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| [pic] Introduction Enzymes are catalysts which speed up a reaction by providing an alternative reaction pathway of lower activation energy. But they do not undergo permanent changes and so they remain unchanged even at the end of the reaction. Many enzymes consist of proteins and non-proteins called the cofactor. The intra- and intermolecular bonds that hold proteins in their secondary
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Potato Enzyme Lab Enzymes are protein catalysts that speed up chemical reaction in living organisms. The purpose of this lab was to; observe the breakdown of hydrogen peroxide toxin by potato’s enzyme catalase‚ determine factors that influence how quickly the reaction takes place‚ and determine factors that influence how well enzymes function. The first test‚ we were supposed to see what would happen when we add sand in hydrogen peroxide. We filled a test tube with 5mL hydrogen peroxide and we added
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Lab Report Enzymes (must be typed) Name: Amanda Gallegos Date: 2/15/15 Section: BIOL 101GL What is the Hypothesis of this experiment? (3 points) -The highest temperature will affect the reaction rate of enzymes. What is the dependent variable? (1 point) -Change of rate/product/color/absorbency. What is the independent variable? (1 pt) -The temperature. Explain in detail the procedure that you followed (including amount of substrate‚ enzyme etc‚ and the whole procedure including incubation times)
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Effects of pH on the Function of Enzymes Rena Mototani Glen Rock High School Advanced Biology 2014-2015 Effects of pH on the Function of Catalase Rena Mototani Problem: How does the pH of a cell affect the function of the enzyme catalase? Introduction In this lab‚ we experimented the effects of pH on the function of the enzyme catalase. Catalase is an enzyme that brings about the reaction by which hydrogen peroxide is decomposed to water and oxygen (Encyclopedia Britannica). The
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Investigate Concentration of Substrate on Catalase enzyme 1. Aim/Prediction. Aim of this experiment is to investigate concentration of substrate on catalase enzyme‚ as well to see how they work in different concentrations of hydrogen peroxide (H2O2). Prediction would be‚ that the filter paper discs that were used‚ will rise up to the surface in a time in the tube of different concentration of hydrogen peroxide. And in different concentrations the time will be different. As higher the concentration
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Title: Catalase enzyme detection Objective: To understand the function of catalase in cells that produce the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of
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HINTS ON WRITING YOUR REPORT Title: Enzyme activity Objective: To investigate the effect of temperature on amylase activity Design principle Background: Amylase activity products? (show the equation) Which factors will affect enzyme activity? How to study the rate of reaction? (e.g. rate of disappearance of substrates or rate of formation of products) Independent variable: temperature of reaction mixture or at which the enzymatic reaction occurs. It can be varied by setting water bath at
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amount of tenderizer did not break down as much as the cup â»3 with the most tenderizer. Conclusion According to this lab it demonstrated that Jell-O does have protein since the enzymes in the meat tenderizer were able to break down the Jell-O and prevent it from setting. This data also shows that the amount of enzymes effects the breakdown of proteins since the cup with the least amount of meat tenderizer set better than the cup with the most amount of meat tenderizerizer CUP NUMBER OBSERVATIONS
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ENZYME STRUCTURE AND FUNCTIONS: Enzymes are biological catalysts. They increase the rate of reactions by a factor of between 106 to 1012 times‚ allowing the chemical reactions that make life possible to take place at normal temperatures Definition of enzyme: A protein with catalytic properties due to its power of specific activation is defined as an enzyme. STRUCTURE Enzymes are proteins their function depends on its complexity. The reaction takes place in a small part of the enzyme
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