Experiment #1 1. At substrate concentration‚ the enzyme is working at “maximum efficiency.” With a concentration at 40‚ it produced 2‚339 products. 2. The maximum velocity of a reaction is reached when the active sites are almost continuously filled. Increased substrate concentration after this point will not increase the rate. The reaction rate increases as substrate concentration is increased. It will soon level off though. 3. When the concentration is at low substrate‚ most of the enzyme
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EFFECT OF TEMPERATURE‚PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature‚Ph and substrate concentration on the rate of enzyme activity. Hypothesis
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USING BLEACHING POWDER A CHEMISTRY INVESTIGATORY PROjECT ANSHUL KUMAR PANDEY XII B [THIS PROJECT LOOKS AT THE TECHNIQUE CALLED STERILIZATION OF WATER USING BLEACHING POWDER WHICH IS USED TO PURIFY WATER AND MAKES IT FIT FOR DRINKING.] INDEX I. Introduction -Need of Water -Purification of Water -Need for a stable purification technique II. Theory -History of Water purification -Bleaching powder and its preparation -Use of Bleaching powder in Sterilization
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PARTIAL REPLACEMENT OF CEMENT WITH MARBLE POWDER A MINI PROJECT REPORT Submitted by; RAHUL-M JAMSHEED .P MUHAMMAD SHANIL .K.P GEO .P. JOSE JAGADEESH .K.A | | | | | | | | In partial fulfilment of the requirements for the award of degree of BACHELOR OF TECHNOLOGY IN CIVIL ENGINEERING DEPARTMENT OF CIVIL ENGINEERING AWH ENGINEERING COLLEGE KUTTIKKATOOR DEPARTMENT OF CIVIL ENGINEERING
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1. A) I would expect the active site of nylonase to consist mainly of polar amino acids with a few nonpolar amino acids as well because the substrate for nylonase is polar overall‚ but has many nonpolar bonds. What makes me think that the nylonase enzyme is polar is that the substrate that would bind to the active site of nylonase has extreme polarity between carbon and oxygen‚ and between hydrogen and nitrogen due to their differences in electronegativity’s‚ but it still has the nonpolar bonds between
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Discussion The primary purpose of this experiment was to determine the optimum temperature range for the activity of the enzyme lactase. Extreme temperatures can have a detrimental effect on enzymes; very hot temperatures can cause the denaturation in the enzyme‚ which is the loss of protein structure. This causes a change in the shape of the enzyme leading to its inability to perform its function. As previously stated‚ the alternate hypothesis read: the optimal temperature range for lactase activity
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Title: “The Effect of Substrate Concentration‚ Enzyme Concentration‚ pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results.
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Enzymes are biological molecules (proteins) that act as a catalyst and help complex reactions occur everywhere in life‚ for example a piece of steak that is being digested into energy. Molecules found at the beginning of the process are called substrates‚ and these enzymes exchange them into differing molecules known as products. Nearly all-metabolic processes in a cell need enzymes in order to function at rates that are fast enough to sustain existence. Those who are lactose intolerant are simply
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An experiment was run to determine which enzyme (pectinase‚ and cellulase or combinations of the two enzymes) maximizes juice production and would be most cost effective. The proposed hypothesis was if the enzyme‚ pectinase‚ is added to apple juice‚ then the more juice will be extracted than if cellulase were added because pectinase holds the cell wall together and if it is separated apart from each other‚ then the more juice would be able to flow out. The experimental data show that during the ten
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amount of HCl that did not react with the calcium carbonate. Apparatus Mortar and Pestle Standard Lab Balance Hot Plates 200ml beaker Measuring cylinder (10ml‚ 100ml) Pipette Burette Conical Flask Bunsen burner Any reagents used in this experiment Eggshell NaOH 1moldm3 HCl 1moldm3 Phenolphthalein Procedure 1. Each student should obtain one egg and the necessary glassware from the side bench. 2. Break the egg into a beaker. Add water to the egg and stir before pouring down the drain
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