Received July 18‚ 1997X A radiochemical enzyme assay for studying cyclooxygenase (COX)-catalyzed prostaglandin biosynthesis in vitro was optimized with respect to both COX-1 and COX-2 activity. The assay can be used to assess the relative selectivity of plant-derived inhibitors on COX-1 and COX-2. Assay conditions were optimized for both enzymes with respect to concentration of cofactors (l-epinephrine‚ reduced glutathione‚ and hematin)‚ activation time (enzyme and cofactors)‚ reaction time‚ and
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the
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Experiments: Doctors‚ Experiments‚ and Results Melissa Anjeanette Edwards POLYTECH High School of Kent County‚ Woodside‚ Delaware Abstract During World War II experiments were done on the prisoners of war in Nazi Germany. Doctors for these camps came in all shapes and sizes including former S.S. Troops‚ Women‚ and a variety of prisoner doctors. The experiments differed as much as the doctors themselves; however they stayed the same in one factor‚ medical curiosity become killing in atrocious
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DISCUSSION: Bromelian added to Gelatin: Bromelian is an enzyme found in pineapples. When Bromelian is added to gelatin it breaks down the protein and does not allow the gelatin to solidify. There are several factors that can cause an enzyme to slow down or to completely stop reacting. For example‚ temperature and pH can effect enzyme activity. Canned pineapple juice and fresh pineapple juice were used to see how the enzyme would react differently. In fresh pineapple juice the Bromelian have would
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the okay to do the experiment‚ I grabbed the girls and went to work. The experiment I picked was the conservation concept‚ where the idea that merely changing the appearance of objects does not necessarily change other key properties. In other words‚ just because I put the same amount of water in a bigger case‚ doesn’t change how much water there is. I predict that both the girls would fail at conservation concept‚ and always
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Experiment Plan By: Ashley Project Question: What size of bouncy ball bounces the highest‚ small‚ medium‚ or large? * I predict the following will happen (my hypothesis): I predict that the smallest ball will bounce the highest since it is the lightest. | * Independent Variable: I will be changing the ball size. * Dependent Variable: The height of the bounce. * Controlled Variable: The height that I will drop the balls at. | The materials and amounts of each that is
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interaction Milgram’s experiment is to study the effect of obedience to authority. Study was performed to determine what factors influenced people to submit to authority and to what extent people conform an order against their conscience despite knowing it causes distress and harm to another person. McLeod‚ S. (1970). 40 male participants between age of 20 to 50 years old from various occupation ranging from unskilled to professional were recruited for the experiment. They were rewarded $4.50
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Are you looking for a fun experiment that you can perform for a psychology class? This experiment on gender and memory is relatively quick and easy to perform‚ which is a bonus if you are short on time and resources. Could gender differences play a role in short-term memory? We often hear women complain that their husbands can’t remember important dates like anniversaries or birthdays‚ but could this phenomenon be due to something like memory differences between men and women. Some previous research
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The Milgram Experiment Outline Topic: The Milgram experiment I) The experiment A) Who was involved with the experiment? B) How they got participants C) What the subjects thought was happening i)Learning Task ii) Memory Study iii) Electric shock for wrong answer iv) “Prods” to continue the shocks D) What actually happened i) It was a test for obedience not memory ii) Vocal response from the victims
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1 English 1013 10/18/10 In nineteen sixty-three‚ Stanley Milgram conducted an experiment on obedience to authority figures. It was a series of social psychology experiments which measured the willingness of the study participants to obey an authority figure who instructed them to perform acts that conflicted with their personal conscience and confronted them with emotional distress. The experiment resulted in twenty-six out of forty of the participants administering the final massive
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