NA transformation of E. coli: The two plasmids were added to individual tubes containing E. coli and one with no plasmids. The three samples of E. coli were heated in a 42°C water bath for 90 seconds to heat shock the bacteria so that the plasmids would be taken up by the E. coli. These samples were then incubated at 30°C for half an hour and then plated on LB agar. Each tube was plated on an LB plate and a LB + ampicillin plate. Ampicillin is an antibiotic that is effective against E. coli‚ both
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reasons.”’ Away With The Cellphone Ban. To City Council: There has been a lot of controversy on your proposal of the ban of cell phones in privately owned businesses‚ which violators will be subjected a fine. I personally do not agree with this proposal for many reasons. I think that this violates our use of communication‚ and safety. Our use of Cellphones are now connected to everyday life task now‚ and they do not have the right to take that away from us. Cellphones are a way of knowing emergencies
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also known as “Daing na bangus” was one of the famous foods in the Philippines and it is one of the delicious and nutritious foods loved by most Filipinos. Fish like bangus contains proteins‚ fats‚ vitamins‚ calcium and iodine that is needed by our body towards a good and a healthy lifestyle. Through the process of deboning milkfish and marinating with vinegar and garlic‚ the product Daing na bangus became known in the country. Dagupan City is one of the famous sources of daing na bangus particularly
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Containerized 200 kW NAS Battery (to be released in 2015) 1. A “Plug & Play” 200 kW NAS Battery Unit (NBU) is under development at NGK. It will: ‐ Expedite shipping‚ installing and relocating multi‐MW NAS installations ‐ Accommodate a broad range of ambient site conditions: e.g. Operational temperature range is from ‐45 to 55C (special type) or –10 to 45C (standard type). Special type for salt‐air coastal environments is available. 2. A new 36 kW NAS module design is required for 200 kW NBUs
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"Narcotics Anonymous is a nonprofit Fellowship or society of men and women for whom drugs had become a major problem. We are recovering addicts who meet regularly to help each other stay clean." After that‚ they directed the attention to a table with the NA book and other books and pamphlets which could be purchased or are for free. After the text was read‚ people celebrated special occasions. New members were welcomed greatly‚ as we had one at the time‚ and also a mother and son who came in later. All
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How cell phones have changed our sense of self Comment0 Share Courtesy of flickr user Bundini Gunjan SinghCognitive Science Examiner Subscribe Follow: Advertisement August 18‚ 2010 Currently‚ more than half of the world uses cell phones as indicated by the mobile penetration rate of 61% that was reached by the end of 2008 (ITU‚ 2009). As cell phones continue to become a part of our daily life‚ there is a need to examine how general population’s attitudes
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LTD MT EDUCARE LTD – SATTEBOARD GEOMETRY ASSIGNMENT - I 20MARKS 1. In the adj fig‚ seg SP ⊥ side YK & seg YT ⊥ seg SK. If SP = 6 ‚ YK =13 ‚YT= 5 and TK= 12 then find :- A(∆SYK):A(∆YTK). 2. In the adj fig.‚ seg DH ⊥ seg EF and seg GK ⊥ seg EF. If DH = 12 cm‚ GK = 20 cm & A(∆ DEF) = 300 cm² then find (i) EF (II) A(∆GEF) (III) A( DFGE) 3. In the adj fig.‚ RP : PK = 3:2 then find the value of : (i) A(∆ TRP) : A(∆ TPK) (II) A(∆ TRK) : A(∆TPK) (III) A(∆ TRP) : A(∆ TRK) 4. In the
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Chapter I Introduction As we all know there has been a great development in technology. This has resulted in invention of many gadgets and cell phone is one of them. Cell phones are considered as an essential item‚ an integral tool necessary for communicating and connecting to families‚ friends‚ and work or even used for emergencies. Based from a research conducted in China on 2009‚ nearly one third of the students there have shown signs of addiction as they felt uneasy and displayed abnormal
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Cell phones ring throughout schools everywhere. Some schools allow students to pick up their phones while other schools prohibit all use of cell phones during the school day. Should teachers allow cell phone usage in the classroom? I say yes‚ yes‚ yes. For the past three years at my high school‚ cell phone usage was not only prohibited‚ but if a cell phone was out‚ any teacher or administrator could take your phone. Students would then get a written detention and if they had it taken away more than
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Anatomy of contemporary GSM cellphone hardware Harald Welte April 16‚ 2010 Abstract Billions of cell phones are being used every day by an almost equally large number of users. The majority of those phones are built according to the GSM protocol specifications and interoperate with GSM networks of hundreds of carriers. Despite being an openly published international standard‚ the architecture of GSM networks and its associated protocols are only known to a relatively small group of R&D engineers
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