Microbiology 215 exam #2 chap 7‚ 11‚ 12 81-90 essay: 10 points Describe the Kirby Bauer Test? Make sure you describe all the key elements. 1. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 2. Place antibiotic disks evenly spaced on the inoculated agar plates and incubate at 37C for 24-48 hours. 3. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis
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MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________ EXPERIMENT 1 TITLE: Observing Bacteria and Blood OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood. PROCEDURES: Using the microscope‚ an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood: After reviewing the section of the manual
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Abstract Development of bacterial resistance to the antimicrobial drugs identified the need to determine a given bacterium’s susceptibility or resistance to a given drug which prompted W. M. M. Kirby and A. W. Bauer to develop a single disk method for susceptibility testing. This experiment used the Kirby-Bauer disk diffusion test to measure the degree to which Penicillin‚ Streptomycin‚ Ampicillin‚ and Chloramphenicol inhibited the growth of the bacterium‚ Proteus vulgaris. The measured zone of resistance
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------------------------------------------------- ------------------------------------------------- EVALUATION OF EFFECTIVITY OF SOAPS AND DETEGENTS AGAINST BACTERIA BACTARIUM EVALUATION
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I measured the spread of the bacteria by testing how resister the bacterial strain is to the antibiotic through the Kirby Bauer antibiotic testing. KB testing or disc diffusion antibiotic sensitivity testing was measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. Windex is a disinfectant that rejects 99.99% of destructive bacteria
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This method was invented by W. M. M. Kirby and his colleague A. W. Bauer in the early 1960’s (Hudzicki‚ 2009). Filter paper disks are used to soak the antibiotic in and then are put in a petri dish with the bacteria. If the antibiotic has an effect on the bacteria then a circle will form around your filter
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CHAPTER 1 Introduction and Its Background Introduction Infectious diseases are one of the main contributors to global mortality and morbidity. Infectious diseases are caused by pathogenic microorganisms‚ such as bacteria‚ viruses‚ parasites or fungi. These diseases can be spread‚ directly or indirectly‚ from one person to another. Vaccines‚ antibiotics‚ and many other advances have lessened the impact of infectious diseases. Unfortunately‚ this has not been true everywhere. It has not been true in
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31 October 2011 Identification and observations of Citrobacter Freundii Introduction Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections‚ diarrhea‚ and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly‚ it is
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Introduction: Chemical methods of control: Antimicrobial drugs‚ involves the use of chemicals to prevent and treat infectious diseases. Pasteur and others observed that infecting an animal with Pseudomonas aeruginosa protected the animal against Bacillus anthracis. Later‚ the word “antibiosis” (against life) for this inhibition and called the inhibiting substance “Antibiotic”. As researchers found out more and more about these chemicals they were able to discover that antibiotics are chemicals
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Microbiology Abstract: The purpose of this lab was to use the Kirby-Bauer method to test the sensitivity of Staphylococcus epidermis of specific areas on a petri dish. The antibiotics were gentamicin‚ novobiacin‚ and penicillin. This resembled a culture and sensitivity test that is preformed at the hospital’s laboratory to find out the resistance of a microorganism. This experiment was meant to use the naked eye and a magnifying glass when necessary. Procedures: This experiment called for prepared
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