inactivated by disinfectants. Enterococci‚ including vancomycin-resistant strains‚ are also susceptible but somewhat less than staphylococci. What are the factors that influence the size of the zone of inhibition of an antibiotic when employing the Kirby-Bauer method? The size of the zone of inhibition is dependent on the difussion rate of the antibiotic‚ the degree of sensitivity of the microorganism‚ and the growth rate of the
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19‚ 2008) ABSTRACT The aim of this study was to investigate the activity of (28%) ethanolic extract propolis (EEP) of [Bazian‚ Pshdar‚ Sharbazher‚ Khormal‚ Kanakawa and Sulaimani (city center)] against (Staphylococcus aureus‚ Staphylococcus epidermidis‚ Escherichia coli‚ Pseudomonas aerugenosa‚ Klebsiella pneumoniae‚ Proteus mirabilis and yeast Candida albicans). Two methods were employed for propolis activity evaluation; firstly by agar well diffusion method using 40µl of propolis extracts
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Identification of Staphylococcus aureus and epidermidis Wendy Heck Bio 175: General Microbiology Fall 2012-11-21 Staphylococcus aureus is the most pathogenic for humans and Staphylococcus epidermidis is part of the normal flora and is of low pathogenicity. Staphylococcus epidermidis and Staphylococcus aureus are two medically important species of bacteria. A culture from the nose and throat was taken to perform whether or not Staphylococcus epidermidis or Staphylococcus aureus were detected.
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In Jane Horack’s article “Staphylococcus epidermidis”‚ S. epidermidis is described as “gram-positive cocci bacteria that are part of the normal flora on the skin and nasal passages.” The article goes on to say that the species was originally named Staphylococcus Albus by microbiologist Rosenback in 1884. When viewed under a microscope S. epidermidis will appear in chains‚ pairs‚ or grape-like clusters (Horak 1). Taxonomically‚ the species S. epidermidis falls in the genus Staphylococcus‚ which is
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diseases in several countries nowadays. There were different procedures used in the experiment: culturing the test organisms‚ extraction of Takip-kohol leaves (pure and boiled extracts) to determine the significant difference between the two and Kirby-Bauer Method. The study conducted was proven to be effective as antibacterial agent. There is a significant difference between pure and boiled Takip-kohol leaves extract. Pure extract of Takip-kohol leaves is proven to be effective as antibacterial agent
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Gram Stain: Gram is an empirical method to distinguish the species of bacteria into two groups Gram-positive and Gram-negative based on the physicochemical properties of the cells. First‚ a smear was prepared by use a sterile transfer loop that been flamed to removes some bacteria from slant agar and placed on the slide; mixed with one drop of water and let air dried. After dried‚ heat fixation the slide by passed the slide over a flame quickly 2-3 times to stick bacteria to the slide. Next‚ the
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In the experiment involving varying pH levels‚ E. faecalis‚ S. epidermidis‚ L. lactis‚ and L. casei were subjected to different pH levels and then were allowed to incubate in order to determine the minimum‚ maximum‚ and optimum pH levels for growth of specific bacterial species. It was found that lower pH levels between 2 and 4 inhibited or promoted little to no growth for E. faecalis‚ S. epidermidis‚ and L. casei. It is evident that these bacteria were resistant to alkaline environments‚ however
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extract was put in reagent bottles and kept refrigerated until use. E. Determination of the Activity of the Extract on the Chosen Bacteria The antibacterial property of leaves extracts obtained from Angelica keskei koidzumi was determined using Kirby-Bauer disk method. Using this method‚ a culture medium‚ nutrient agar‚ was uniformly and aseptically inoculated with the test organism and then filter paper discs soaked in 10 ug/mL of ashitaba extract are placed on the medium. Incubation was done for
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CHAPTER 4 RESULTS AND DISCUSSION 4.1. Angelika keiskei (Ashitaba )crude non-polar extract The crude non-polar extract from the plant Angelika keiskei (Ashitaba) was tested for the anti-bacterial property by using the Kirby – Bauer Disk Diffusion test. There were four 25uL of Ashitaba non-polar extract with different concentrations used which were 12.5 %‚ 25%‚ 50% and 100%. In line with this‚ respective positive controls of the two specimens and negative control were also used. The results
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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