in Table 4. Bovine serum albumin (BSA) was diluted with distilled water. The absorbance of each sample were measured using spectrophotometer at 595nm. Standard protein curve was plotted according to the concentration of protein where the x-axis and y-axis represent protein standard concentration and absorbance (595 nm) respectively. 3.10.2 Protein Determination The treated and untreated samples were measured using Bradford assay. 10µl of the samples were made up to 100µl with distilled water
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QUANTITATIVE DETERMINATION OF COPPER (II) CONCENTRATION BY SPECTROPHOTOMETRY D.DEL PRADO1‚ J. BELANO1‚ M.MAHUSAY2‚and M.FRANCISCO2 1 DEPARTMENT OF FOOD SCIENCE AND NUTRITION‚ COLLEGE OF HOME ECONOMICS 2INSTITUTE OF CHEMISTRY‚ COLLEGE OF SCIENCE UNIVERSITY OF THE PHLIPPINES‚ DILIMAN‚ QUEZON CITY 1101‚ PHILIPPINES DATE SUBMITTED: 12 MARCH 2013 DATE PERFORMED: 7 MARCH 2013 ------------------------------------------------- ------------------------------------------------- ABSTRACT -------------------------------------------------
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In this folio task‚ the impact of drug absorption and blood stream concentration‚ on the human body‚ will be observed. The rate of absorption and clearance of the drug‚ relies on many factors. The drugs blood stream concentration‚ time of administration‚ absorption rate‚ clearance rate and the effect of certain parameters‚ will be calculated. This can be completed through using a surge function‚ calculus (derivatives) and straight line graphs (). Graphing technology on the graphics calculator
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13‚ 2013 Determining the Concentrations of Red Dye in Sodas Abstract The use of red dye #40 is common in various soft drinks today. The labels on these beverages do not specify how much dye we are consuming. We did this experiment to find out which soda uses the most dye. Using a spectrophotometer‚ we measured how much light is absorbed by various known concentrations of red dye. After collecting this data‚ a standard curve was made that correlated the concentration of red dye #40 to its absorbance
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conclusion is that the higher salt concentration in the solution the greater the decrease in mass of the potato will be‚ the graph I have drawn supports my conclusion. The 10% salt concentration shows that on average the potato loses 2.6% of its overall mass. 20% salt concentration shows a further decrease in mass this time it is an average of 13% overall mass decrease. 30% salt concentration shows an average decrease of 18.8% in overall mass. The 0% salt concentration actually shows a mass increase
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The effect of salt concentrations on the mass of the pear cubes ‘Pyrus’ after being soaked in water Aim: The effect of salt concentrations on the mass of the pear ‘Pyrus’ pieces shaped as cubes of 1cm. Background: Osmosis is the net movement of water molecules through a semi-permeable membrane from an area of higher water potential to an area of lower water potential. Water makes up to 70-90% of living cells and cell membranes are partially permeable membranes. Dissolved substances attract
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Noel Arackal 1.25.16 Bisc 4930 Experiment No. 1 – Varying Extracellular Concentration Introduction: This experiment sets out to determine the tonicity of solutions with varying concentrations of sodium chloride on the red blood cells of sheep by measuring the transmittance of a red blood cell/NaCl solution with a spectrometer. This is done in order to study the effects of solutions containing varying levels of tonicity to red blood cells. Tonicity describes what happens to a cell when it is
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The Effect Of Enzyme Concentration On Enzyme Activity The pancreatic duct in individuals who have cystic fibrosis frequently becomes blocked‚ reducing or preventing the release of pancreatic enzymes into the small intestine. The aim of this activity is to investigate the effect of a reduction in enzyme concentration on the rate of reaction‚ in this case the breakdown of protein by protease enzymes. Aim – Milk powder contains a white protein called casein. A white suspension of
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investigating how the rate of reaction differs when we change the concentration of Hydrochloric Acid whilst reacting with Magnesium. The rate of reaction is explained by the Collision Theory. This theory explains how various factors affect the reaction rates and how chemical reactions occur. The 4 factors of the Collision Theory are: • Temperature • Concentration • Surface area • Catalyst I am investigating how different concentrations of acids affect the rate of reaction. However I must control
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Experiment 2: Estimation of protein concentration Name: Wong Jiun Hao ID: 00000011136 Cohort: BM114 Module: Biological Science Table of Contents Title 1 Table of Contents 2 Introduction 3 Objectives 4 Procedure 4 Results 5-6 Discussion 7-8 Conclusion 9 References 9 Introduction The concentration of protein in a sample can be obtained by using the Bradford protein assay method. The method requires a spectrophotometer
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