LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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a Beam Objective 1. To examine the stresses at various positions of the beam under a constant load of pure bending. 2. To determine the curvature of deflection of the beam. Introduction 1. Pure Bending and Nonuniform Bending When analyzing beams‚ it is often necessary to distinguish between pure bending and nonuniform bending. Pure bending refers to flexure of a beam under a constant bending moment. Therefore‚ pure bending occurs only in regions of a beam where the shear force
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The spring constant Manuel Cereijido Fernández – UO237242 PL-4 -----------------------------------------------------------------------------------1. Objectives The main objective of this experiment was to determine the spring constant using the dynamic method. 2. Theoretical fundamentals On the one hand‚ when a spring oscillates‚ the movement which describes can be classify as a simple harmonic motion. Therefore‚ its position‚ velocity and acceleration equations respect the time will
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directly proportional to the surface areas and the inverse of the separation of the two conductors. The dielectric constant of the non-conductor is also affecting the capacitance. FIGURE 1 Capacitor symbol For an ideal capacitor‚ the capacitor current iC is proportional to the time rate of change of the voltage across the capacitor: Where C is the proportionality constant and is known as capacitance. (b) Inductor Inductor is an electrical passive device for storing energy in the form
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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ends of the cart. The data obtained supported that simple harmonic motion obeys Hooke’s law as the spring value constants were all positive and the graphs displayed a positive slope. The percent discrepancy obtained for one spring was 14.66% and the percent discrepancy for two springs was 0.375%. Objective: The physics concept being studied from this experiment is the spring constant and simple harmonic motion. Equipment Part 1 Two nearly identical springs Long rubber band Support stand with
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
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Abstract: The Enzyme Lab results where when the liver was frozen‚ its reaction was fast‚ and when it was hot‚ it was slow‚ and the liver that was at room temperature reacted slowly to medium. Introduction: The Enzyme Lab is to conduct investigations to determine the most favorable conditions for the most efficient enzyme activity. Variables to be used testing include temperature‚ pH values and surface area. Enzymes are proteins that speed up the rate of chemical reactions‚ which would otherwise
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