The prediction for the effects of temperature on the enzyme activity was that the reaction’s rate would increase as the temperature increased‚ until they go over the optimum temperature where the enzymes denature and the reaction’s rate quickly drops to zero. At 5 degree C the rate is 0.00059mole PNP/min. This then increases to 0.01031mmoles PNP/min at a temperature of 50 degree C. The rate then drops drastically to -0.00215moles PNP/min. This point is where the enzymes have been denatured and
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Studying the Rate of Reaction of catechol oxidase and how it affects pH levels Introduction: In this lab‚ we studied the activity of an enzyme that is found in fruits and vegetables called catecholase or catechol oxidase. An enzyme is a protein molecule that is a catalyst. Catechol oxidase is the enzyme in fruits and vegetables that turns them that undesirable brownish color; also commonly referred to as bruising or bruised. When walking through your regular grocery store and you find yourself in
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Introduction: The purpose of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The reaction followed is given below: Tyrosinase³ Enzyme Pyrocatechol Hydroxyquinone Oxidation/Reduction Pink ³ Brown E+S + [ES] = E+P Enzyme Reaction Hypothesis: If there is an increase in enzyme concentration‚ an increase in reaction temperature‚ or an increase in buffer pH‚ then greater
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Lab report April 14‚ 2013 Abstract: In this article‚ we will experiment on the significant in strength of the enzyme by using three different test tubes and measuring the amount of product they give off. To determine this we are going to test the amount of color absorbance by using a special tool to help us understand our results. We will see how our end results show the effect of the amount of concentration we apply to each test tube. The results would be shown by the support of two graphs
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Observation Zinc Shot: Silver color‚ some pieces are shiny while the majority is dull. Tear shaped pieces (round at base with pointed top). No odor. Zinc Granular: Silver in color‚ semi shiny‚ flaky appearance. No odor. Zinc Sheet: Sheet of metal‚ flat pieces that are about 3 inches wide & 2 inches in length & 0.02 inches in height. Each piece has a dull side and a shiny side. Silver in color‚ the dull side has a darker shade of gray. No odor. Zinc Powder: Gray very fine powder. No odor
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(a) Define the term equilibrium potential and use the Nernst equation (see Appendix) to show how the equilibrium potentials for both sodium and potassium are calculated. Equilibrium potential is the potential of the membrane when there is no net flow of ions from one gradient to the other gradient. The ions are equal and are opposite of each other but not moving from one side to the other. Sodium: Ena=2.303((8.31 J m-1 K-1)(310K))/((1(= 9.65 x 104 c m-1))(log(150/15))= 61.54 mV Ek=2.303((8
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the concentration was gradually increased starting at .1ml to .2ml and finally to .4ml. Guaicol is used in the reaction as in its oxidized form it takes a brown color which in the concentration it can be measured by the spectrophotometer [cite pre lab]. The Temperature was changed 4 times in Activity 3 as we used a water bath at 60 C‚ a body
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BIOL380 Genetics Lab Report 1Hardy Weinberg Equilibrium Note on late reports: You have almost one month to complete this lab report. I will NOT accept late lab reports. You must turn the report in at the start of class (that means 1:00 AM sharp!) December 1‚ 2010. If you come to class late‚ I will not accept your lab report. Please consider e-mailing me your report before you come to class to make sure it is not late. Here is what I expect you to cover in your report: 1. Introduction:
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Purpose. The purpose of this lab was to understand equilibrium. To do this‚ you must find the equilibrant of the resultant of three vectors‚ both mathematically and graphically and test the results. Procedure: A) Put the weights necessary for each of the vector forces on each hook. B) Set the wheels of the force table at the proper angles‚ including the calculated equilibrant. C) When placing the hooks on the wheels‚ be careful to hold the table in place so it does not flip over. D) To test
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Enzymes are important components of life‚ facilitating reactions that are necessary for an organism to live. Enzymes can be very specific to what environment they function best in1. Numerous environmental impacts were tested for the enzyme peroxidase which catalyzes the decomposition of hydrogen peroxide. The basic decomposition reaction was carried out first without any environmental alterations. The hypothesis for this reaction was supported. The enzyme caused the amount of absorbance increase
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