EXPERIMENT 5 SEPARATION OF A MIXTURE INTO ITS COMPONENTS BY FRACTIONAL CRYSTALLIZATION Introduction: Fractional crystallization is one of the experimental techniques used to separate or purify mixture. Fractional crystallization makes use of the differences of solubility to separate the components of a mixture. Goals: Applying fractional crystallization to separate a mixture of salicylic acid and copper sulfate pentahydrate into its components. Calculate the percent of salicylic
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Effect of enzyme concentration on rate of reaction Research question: is there a relationship between catalase concentration and the rate of reaction? Hypotheses: if the enzyme concentration is increased then the rate of reaction will increase. If the enzyme concentration is decreased then the rate of reaction will decrease. Controlled: temperature Independent: % catalyze concentration ( Dependent: rate of reaction (mm/s) Materials: Mortar and pestle Graduated cylinder Distilled
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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The Difficulty Limited/Extensive choices pose: Its Effect on motivation and Satisfaction The Difficulty Limited and Extensive choices pose: Its Effect on motivation and Satisfaction Teagan. R. Donnelly 214282075 HPS121 Psychology B: Individual and Social Development The Difficulty Limited/Extensive choices pose: Its Effect on motivation and Satisfaction Abstract The conducted study is based on personal choice in limited and extensive choice experimental
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Purpose: To observe epithelial tissue by using a microscope to compare prepared slides. Introduction: Epithelial tissue covers the whole surface of the body. It is made up of cells closely packed and ranged in one or more layers. This tissue is specialized to form the covering or lining of all internal and external body surfaces. Epithelial tissue can be divided into two groups depending on the number of layers of which it is composes. Epithelial tissue which is only one cell thick is known as
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1. OBJECTIVES (a) To determine the equivalent circuit parameters of a 3-phase squirrel-cage induction motor from laboratory test data. (b) To determine the performance characteristics of an induction motor under load conditions. (c) To control the no-load speed of an induction motor. 2. INTRODUCTION 2.1 Three-phase squirrel-cage induction motors An induction motor is a transformer with a rotating secondary winding. It has two essential components: an outer stationary stator and an
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Synthesis of Butyl Benzoate Using Phase Transfer Catalysis The objective of the experiment is to synthesize the butly benzoate by nucleophilic substitution and characterize it by IR spectroscopy. The percent yield of the final product is determined after the synthesis. Procedures: 2.0 mL of 1-bromobutane‚ 3.0 g of sodium benzoate‚ 5.0 mL of water‚ 4 drops of Aliquat 336‚ and a boiling stone were placed in a 50mL round-bottomed flask. The reaction mixture was refluxed for 1 hour and the flask
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CHM 3411 – Problem Set 6 Due date: Wednesday‚ March 23rd. Do all of the following problems. Show your work. 1) Consider the cyclic molecule C8H8‚ the eight carbon analogue to benzene. a) Write the secular detrerminant corresponding to the pi-bonding in C8H8. b) Using the secular determinant‚ the following energies are found for the pi-bonding molecular orbitals: 1 = + 2 2 = + 1.41 (two states) 3 = (two states) 4 = - 1.41 (two states) 5 = - 2 Give the electron
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Introduction: During this laboratory exercise will identify by the graph given which is isotonic‚ hypotonic‚ or hypertonic. The kidneys are a pair of fist-sized organs located outside the peritoneal cavity on each side of the spine. The kidney is a highly specialized organ that maintains the internal environment of the body by selectively excreting or retaining various substances according to specific body needs. The process of urine formation and adjustment of blood composition involves three
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Questions and Hypothesis: Seed germination‚ plant growth‚ and sprouting of leaves are affected by several factors: supply of nutrients‚ water‚ exposure to sunlight‚ and conditions of surroundings. Due to certain stimuli in the surroundings plant “hormones” cause plants to behave in ways that ensure the most efficient use of resources while preserving the most energy. One stimulus to consider‚ is the exposure of plants to sunlight; when plants are just sprouting and are underground‚ the sti
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