EXPERIMENTAL PROCEDURE: 1. 2.07 g of the mixture of naphthalene and benzoic acid was weighed accurately on a balance. The mixture was then dissolved in 40 ml dichloromethane and the solution was poured into a separatory funnel. 2. 20 ml of aqueous sodium bicarbonate solution was added to the dichloromethane. A stopper was placed on the separatory funnel and was shaken and pressure was frequently released. The 2 liquids separated into clear layers and the lower layer (dichloromethane layer) was
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ISOLATION AND CHARACTERIZATION OF A STEM CELL POPULATION FROM MENSTRUAL BLOOD [pic] Thesis submitted to The American University of Athens‚ to the Faculty of Science‚ in fulfillment of the requirements for Doctorate degree in Biomedical Science June 2011 Acknowledgements: This dissertation would not have been possible without the guidance and the help of several individuals who in one way or another contributed and extended their valuable assistance in the preparation and
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Extraction of DNA from Calf or Hog Thymus/Isolation of Yeast RNA I. Abstract Nucleic acids may be divided into two groups RNA and DNA. DNA contains almost all the genetic information while RNA serves as the bridge between the DNA and proteins. Study of both DNA and RNA initially involves proper extraction/isolation. The storehouse of eukaryotic DNA is the nucleus (and in the mitochondria)‚ so experimentally‚ DNA is extracted from tissues that have a high nuclear to cytoplasmic mass ratio‚ such
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the themes of loneliness and isolation explored in “Of Mice and Men”? Born in 1902‚ John Steinbeck‚ author of ‘Of Mice and Men’‚ set his book in the 1930’s. The novel revolves around two hard-working men dependant on one another during the Great Depression‚ a time when most were alone. Many themes are explored in the novel such as racism‚ loneliness‚ disability‚ isolation and friendship. This essay will be talking about how the themes of loneliness and isolation are explored in ‘Of Mice and
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Isolation of Eugenol from Cloves by Steam Distillation and its Identification by Infrared Spectroscopy Eim A. Chemist CHEM 303 June 16‚ 2005 INTRODUCTION “Essential oils” are the volatile components associated with the aromas of many plants.1 In this experiment‚ the essential oil eugenol (the main component of oil of cloves) will be isolated from ground cloves using the technique of steam distillation‚ which is often used to isolate liquid natural products from plants.2 The principle
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Isolation and identification of pathogenic bacteria associated with frozen mackerel fish (Scomber scombrus) in a humid tropical environment Eze‚ E. I. 1 ‚ Echezona‚ B. C. 1 * and Uzodinma‚ E. C. 2 1 Department of Crop Science‚ University of Nigeria‚ Nsukka‚ Nigeria. 2 Department of Science Laboratory Technology‚ University of Nigeria‚ Nsukka‚ Nigeria. Accepted 8 January‚ 2010 Aquaculture products can harbour pathogenic bacteria which are part of the natural micro-flora of the environment. An in-vitro
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Experiment 3: Isolation of Chlorophyll from Spinach and Chromatography Performed February 14th and 16th By Kyle Herzog Organic Chemistry 344 Section 807 Spring 2012 Objective: The purpose of the experiment is to extract pigments from spinach leaves and separate them by column and thin layer chromatography‚ determining Rf values for the pigments. Equations: Rf= Distance travelled by a given compound/Distance travelled by a solvent solution 1. Crude- 4.25/5.5= 0.773 2. Carotene-
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This essay will compare and contrast the feeling of isolation faced by the characters in Marilyn Chin’s Moon and Murakami Haruki’s Super-frog Saves Tokyo. The way they adopted to regain connection to the society and to reduce the feeling of loneliness and isolation would also be examined. A comparison would also be made between isolation caused by inter-ethnic factors and intra-ethnic factors. Moon was the first story of Marilyn Chin’s book‚ Revenge of the Mooncake Vixen. It was a Chinese American
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English Controlled Assessment Question: Explore the methods Steinbeck uses to demonstrate the effects of isolation on people in Of Mice and Men “You’re all scared of each other that’s what.” This quote is one of the main points of John Steinbeck’s novel‚ Of Mice And Men‚ the effect of isolation on human beings‚ and specifically the culture of our nations that forces people to be isolated. Steinbeck almost immediately sets the course of the book with his setting‚ the 1930’s depression was an
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The movie‚ Gran Torino‚ is about an old war veteran who has just lost his wife. He is alienated from his family and lives alone with his dog. He suffers from a terminal illness but doesn’t tell anyone. When one of his neighbors‚ Tao‚ is pressured into trying to steal Walt’s Gran Torino he gets caught by Walt‚ and Tao’s mother makes him work for Walt in order to make up for trying to steal his car. The more Tao works for Walt‚ the more they begin to respect each other. Eventually‚ Walt helps Tao become
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