Experiment # 4a Title: Determination of the Hardness of Water from a Waterfall Aim: To determine the molarity of EDTA and to determine the hardness of water by measuring the concentrations of calcium and magnesium in a water sample by titration. Abstract: The aim of this experiment was to determine the molarity of a sample of EDTA and then to use this sample to determine the hardness of a sample of water. This was done using a titrimetric method. This was standardized using calcium chloride.
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(large)‚ boiling tubes with stoppers x 12‚ boiling tube rack‚ scalpel‚ a pair of tongs‚ 2 pipettes (± 0‚1ml)‚ balance (±0‚1g)‚ 2 beakers‚ marker‚ sucrose solutions (0.0M‚ 0.1M‚ 0.2M‚ 0.3M‚ 0.4M‚ 0.5M)‚ distilled water‚ filter paper‚ cork borer and Erlenmeyer flask with stopper. Teacher’s instructions: ▪ The initial mass of every piece of potato should be similar and even. ▪ In the graphs‚ instead of joining all the points‚ having as a result a gorge line‚ we should draw the best fit line‚ thus having
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acetic anhydride in an Erlenmeyer flask. 4. Add 0.5 ml of concentrated sulfuric acid. 5. Heat for 30 minutes at a temperature of 70o C‚ slowly stirring the mixture gently. Use the hot water bath under the fume hood‚ and insert a thermometer in the flask to monitor the temperature; make sure the temperature does not exceed 70o C. 6. Allow the flask to cool down to around 50o C. 7. Measure 750 ml of cold tap water. 8. Pour the cold water into the mixture. 9. Prepare a Buchner flask. 10. Insert the Buchner
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is a soft metal‚ it will most likely bond to Sulfur because they are both soft. 20A Preparation of CuCl2~2DMSO Experimental We prepared the sample by synthesizing Copper Chloride with DMSO. 0.160 grams of CuCl2~2DMSO was added to a 10 mL Erlenmeyer flask with a magnetic stir bar. Than 1 mL of absolute ethanol
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pieces in order to expose fresh metal by removing possible magnesium oxide that was on the surface of the turnings. The turnings were added to the round bottom flask as well as an iodine crystal‚ which facilitates the reaction by cleaning the surface of the magnesium metal. Bromobenzene and anhydrous diethyl ether were then added to the flask. Anhydrous ether was used because at this point‚ no water was to enter the reaction. The reaction was triggered by swirling and the heat of a human hand. From
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EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8
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Lab #24- Preparation 4-Methylcyclohexene Introduction: The goals in this lab were to have a reaction occur with 4-methylcyclohexanol and an acid catalyst to form our product of 4-methylcyclohexene via an E1 reaction. This reaction is accomplished by removing the –OH group on 4-methylcyclohexanol via dehydration and to have a double bond form via a loss of a hydrogen on a β-Carbon. Many techniques and skills were developed in this lab. Among them were dehydration‚ isolation‚ drying
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the titrant allows for calculation of the chloride concentration. Materials: volumetric pipettes‚ tubes‚ 250 mL Erlenmeyer flasks‚ 50 mL buret‚ 16.7 g/1L of AgNO3‚ CrO4 solution. Weigh out the sample (NaCl + KCl mixture) into a dry tube in a range of 0.150 g to 0.250 g. Dissolve the salt with water to 10 mL/15 mL/20 mL. Pipette a 5 mL aliquot of diluted salt into a 250 mL flask and add distilled to 100 mL mark. Then‚ add 1 mL of CrO4 indicator to the solution. Titrate the sample with silver
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Date of experiment: September 3rd‚ 2013 Organic Chemistry II – CHLB330 Name: Symone MoxeyLab Partner: Lynden Cooper Isolation of caffeine from tea leave Abstract:- Introduction:- Caffeine is probably the most commonly used addictive drug. This drug is found in a number of things ingested by people. It belongs to a group of nitrogen bases called purines. Because caffeine possesses these base-like properties‚ it is described as an alkaloid. They usually taste bitter and often are physiologically
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the dichloromethane. The mixture resulted into to two layers with the upper layer being the aqueous solution while the lower contained the organic substance. After discarding the upper layer‚ the organic layer extracts were transferred into the Erlenmeyer flask containing the anhydrous sodium sulfate and was decant on a weighed evaporating dish under the hood. After which‚ it was evaporated to dryness. The residue was weighed and the percentage yield was calculated. The residue for this experiment weighed
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