Glucose oxidation by Gluconobacter oxydans: characterization in shaking-flasks‚ scale-up and optimization of the pH profile Received: 20 September 2002 / Revised: 3 December 2002 / Accepted: 6 December 2002 / Published online: 26 February 2003 Springer-Verlag 2003 Abstract In this study‚ the advantage of a novel measuring device for the online determination of oxygen and carbon dioxide transfer rates in shaking-flasks is reported for glucose oxidation by Gluconobacter oxydans. In this fermentation
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round bottomed flask‚ Hirsch funnel and filter flask. PROCEDURE: 1. Take a 50-mL round-bottomed flask and a small condenser and set up a reflux apparatus. Arrange a ceramic fibre-centered wire gauze on an iron ring such that it remains two inches above the burner. Position the clamps on flask and condenser accordingly. 2. Weigh a 2.0-g of impure acetanilide and keep just pinch of it aside for melting point determination. Add rest of it to the 50-mL flask by temporarily
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the flask is 159mL. TRIAL #3 Qualitative • The solvent is clear liquid that has a water-like consistency. • The unknown solvent is the sample #3 • After the heating‚ the organic solvent is still clear. • As the heating continues‚ the liquid portion of the solvent gradually starts to disappear. Noticeable precipitation or significantly visible chemical reactions were not observed. • Flask is empty and the liquid is no longer present in the flask. • When the cold water was ran over the flask to speed
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extracted it is then compared to the nutritional facts amount per serving on the tea bags box. Supplies: Glassware: 1. 50.0 m L graduated cylinder 2. 150.0 mL beaker 3. 2-glass stirring rod 4. 6-test tubes 5. 2-glass pipettes 6. 2-50.0 mL Erlenmeyer flasks 7. glass funnel Hardware: 1. hot plate (Corning PC-420P) 2. electronic scale (S/N: 8330280171) 3. stapler with at least 2 staples 4. test tube rack 5. hot plate tile 6. refrigerator 7. 7-rubber stoppers 8. centrifuge (Becton Dickinson;
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molar mass. This study will apply the ideal gas law in tandem with the concept of volatile liquids and basic molar mass related stoichiometry to identify the molar mass of an unknown liquid. A small sample of the unknown liquid was but into Erlenmeyer flask and vaporized in a water bath. Measurements of mass‚ pressure‚ temperature and volume were taken in order to calculate a molar mass of 78.09 gmol-1 . Calculated results from other groups who followed the same procedure included 78.61 gmol-1 and
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accepted concentration of HCl. It illustrates that we made some errors during the lab‚ the possible causes are water left in the flask and buret which makes the concentration of NaOH decreased‚ the inaccurate measurement of the volume of NaOH and HCl used. Questions Why does the pink color‚ which forms at the point where the NaOH comes into contact with the solution in the flask‚ disappear more slowly near the endpoint? The reason is that the Phenolphthalein indicator slightly dissolve in HCl solution
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organic layer into a clean‚ dry 50 mL Erlenmeyer flask using a disposable glass pipette leaving emulsion behind. Repeat the previous two steps for three more times and remove the bottom layer‚ add it to the same 50-mL Erlenmeyer flask. Carefully swirl the organic layers in the 50 mL Erlenmeyer flask. Dry Erlenmeyer flask either by pipette
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Purpose To check whether mass is gained or lost during a Chemical reaction. Hypothesis Equipments and Materials • Eye protection • Test tube • 250 ml Erlenmeyer flask and stopper • Weight Balance • Antacid tablet • Dilute solutions of o Sodium Hydroxide‚ NaOH(aq) o Iron(III) Nitrate‚ Fe(NO3)3 • Funnel • Cylinder Procedures 1. Put eye protection on for safety purposes. Part A: Reaction between Iron(III) Nitrate and Sodium Hydroxide. 2. Take two cylinders and fill one (full)
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Abstract: The goal of this experiment was to synthesize aspirin. In this experiment aspirin‚ also known as acetylsalicylic acid‚ was synthesized from salicylic acid and acetic anhydride. In the reaction the hydroxyl group on the benzene ring in salicylic acid reacted with acetic anhydride to form an ester functional group. This method of forming acetylsalicylic acid is an esterification reaction. Since this esterification reaction is not spontaneous‚ sulfuric acid was used as a catalyst to initiate
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produced from the reaction between the 1.0 M hydrochloric acid and the calcium carbonate from a reaction chamber into a flask containing water. This experiment will be performed by placing about 3.0 grams of calcium carbonate chips into a flask containing 35 mL of 1.0 M hydrochloric acid at one of the targeted temperatures. This flask is called the reaction chamber because it is the flask that contains the actual occurring reaction. The reaction between calcium carbonate and hydrochloric acid creates carbon
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