Chapter 22: Oral and Gastrointestinal Diseases Question Type: Multiple Choice 1) Feces are composed about 50% by weight and volume of bacteria. Most of these are species of ________. a) Salmonella b) Escherichia c) Bacteroides d) Streptococcus Answer: c Difficulty: Medium Learning Objective 1: LO 22.1 Review the anatomy of the digestive tract as it pertains to microbial defenses. Section Reference 1: Section 22.1 Components of the Digestive system 2) Streptococcus form ________ ‚ polysaccharides
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due to the single celled nature of bacteria. In this lab‚ the engineered pGLO plasmid is integrated into E. Coli bacteria‚ and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia coli with plasmids‚ 1983). To see the reaction of this plasmid on the cells‚ bacteria treated with the plasmid were grown on two separate agar plates containing LB nutrient broth and ampicillin‚ and another containing LB nutrient broth‚ ampicillin
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Aseptic Technique and Streak Plates Purpose The purpose of this exercise is to learn aseptic technique procedures and there importance and also to learn to isolate colonies using the streak plate technique. Introduction Bacteria are inoculated (introduced) and cultured (grown) in the laboratory for test studies to determine their morphology (the shape‚ size‚ arrangement‚ and internal structures) and pathology (ability to cause disease). Inoculation has to be performed without adding other microbes
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Introduction to Microbiology Laboratory report №10 Physical factors affecting growth microbes: Temperature‚ pH and oxygen requirement. Student: Temirlan Aitbekov Lab partner: Kanat Sadykov Instructor: Alessandra Clementi‚ MD‚GP Lab date: 7/11/14 Due date: 14/11/14 Nazarbayev University Abstract: This experiment is directed
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For the completion of this experiment the procedures were guided with the Rainbow Transformation1 lab manual. An Escherichia coli bacterial reference plate was used to obtain colonies which were resuspended into a CaCl2 solution that was previously kept on an ice bath. The rainbow transformation mixture containing the plasmid DNA was then added to half of the E. coli cells. These cells were later placed into a water bath set to 42ºC and “heat shocked” to promote the entrance of DNA into the cells
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type written‚ including the trees. Please be aware of spelling‚ sentence structures‚ and grammar. Always capitalize genus and use only lowercase for species and italicize them. After the first use you may abbreviate the genus (E.G. 1st mention= Escherichia coli ( E. coli after it was spelled out once) This report will take work but we will work on the data collection for it over several weeks. Good notes are going to be key for your report construction! A well done report ALSO reviews a lot of important
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References: Bergmans‚ H. E.‚ Van Die‚ I. M.‚ & Hoekstra‚ W. P. (1981). Transformation in Escherichia coli: Stages in the Process Panja‚ S.‚ Aich‚ P.‚ Jana‚ B.‚ & Basu‚ T. (2008). How does plasmid DNA penetrate cell membranes in artificial transformation process of Escherichia coli? Molecular Singh‚ M.‚ Yadav‚ A.‚ Ma‚ X.‚ & Amoah‚ E. (2010). Plasmid DNA Transformation in Escherichia coli: Effect of Heat Shock Temperature‚ Duration‚ and Cold Incubation Wilfinger‚ W. W.‚ Mackey‚ K
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Molecular Biology Lab Report Payton Jackson Introduction In this lab‚ I am going to use antibiotic-resistance plasmids to transform Escherichia coli. Materials For this lab you will need the following: LB Agar Petri dishes Beakers Test tubes CaCl2 solution Sensitive E. coli (-ampR) amp plasmids ampicillin -amp cells Water bath to heat shock cells A freezer to incubate cells Process Step 1: Wash hands and sanitize lab setting. This will prevent anything reacting with a
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Available online at www.sciencedirect.com Polyhydroxyalkanoates: bioplastics with a green agenda Tajalli Keshavarz and Ipsita Roy Production of polyhydroxyalkanoates (PHAs) has been investigated for more than eighty years but recently a number of factors including increase in the price of crude oil and public awareness of the environmental issues have become a notable driving force for extended research on biopolymers. The versatility of PHAs has made them good candidates for the study
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selected and subjected to qualitative tests for identification. It is suggested that culture #72 is an example of Serratia marcescens and Micrococcus luteus. There were ten bacteria species possibilities: Stahylococcus epidermidis‚ Bacillus subtilis‚ Escherichia coli‚ Serratia marcescens‚ Sarcina lutea‚ Pseudomonas fragi‚ Micrococcus luteus‚ Alcaligenes faecalis‚ Clostridium sporogenes‚ and Micrococcus roseus. There were several qualitative tests that could be conducted to determine the identity of the
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