then starved of nutrients (glucose and nitrogen) – PHB is produced as an energy store for the bacteria. - In 1980’s Maddison University (Virgina) successfully cloned the 3 genes of A. Eutrophus that control PHB production and transferred them to Escherichia coli – an easier bacteria to work with allowing easier manipulation of the polymer depended on the need. - In 1990’s the 3 genes were cloned into sugar beets and turnips – much larger scale production of polymer. Work continuing (with Monsanto
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Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene Introduction: In this experiment‚ a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can
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bacteria and abruptly change carbon source with C12. Then I will collect four samples in different time and analyze the results from centrifugal by ultraviolet absorption. According to the hypothesis above‚ I design the experiment below. Firstly‚ Escherichia coli B will grow 14 generations at 36° C. with aeration in a glucose salts medium which is the only carbon source and labeled with C13. Then I will abruptly change the only carbon source from labeled with C13 to C12. At the same time‚ I also add
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dominant in the gut microflora of breast fed infants and also he observed the clinical advantages in using Bifidobacteria in treating infant diarrhoea. Alfred Nissle‚ German professor‚ during an outbreak of shigellosis in 1917‚ isolated a strain of Escherichia coli from faeces of a soldier who was not infected by the disease‚ antibiotics were not much developed during those times and Alfred Nissle used this identified strain of E.coli for the treatment of acute gastrointestinal salmonellosis and shigellosis
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No. Investigatory Project Title Year 1 A Comparative Study on NPK Content and Production on Various Castings Produced from Animal Manure using Vercomposting 2000 2 Acacia Leaves as Effective Filters for Vehicle Exhausts 2002 3 An Antimicrobial Ointment Manufactured from Kamantigue (Impatiens balsamina) Leaves Extract A Comparison with Miconazole Nitrate 4 Antibacterial Activity of Janitor (Hypostomus plecostomus) Fish Oil Extract Against Escherica Coli and Staphylococcus Aureus 2013 5 Baby
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the streak plate procedure‚ used to isolate pure colonies of bacteria‚ and viable plate count methods. The latter involves serial dilution and spread plating of bacteria on agar plates. Materials required per pair • One 10 ml liquid culture of Escherichia coli BL21 (see prior preparation) • A sample of Yakult (approximately 5 ml) • Marker pens to label plates & bottles • Sterile plastic loops for streaking bacteria (up to 20 per pair) • Sterile plastic spreaders for spreading bacteria (4
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MASINDE MULIRO UNIVERSITY OF SCIENCE AND TECHNOLOGY P.O. BOX 190-50100 KAKAMEGA FACULTY OF SCIENCE DEPARTMENT OF BIOLOGICAL SCIENCES COURSE TILTE: INDUSTRIAL/FIELD ATTACHMENT COURSE CODE: SBL326 NAME: PAMELA K. MUKWEYI REG. No.: BTE/0517/08 DURATION: 9TH MAY – 20TH JULY 2012 SUBMISSION DATE: ATTACHMENT PLACE:CENTRE FOR MICROBIOLOGY RESEARCH- KEMRI Scope/purpose The Industrial Attachment program fulfils part of the requirement in pursuing the degree
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Gene Expression Introduction: Escherichia coli are capable of using lactose as their sole carbon source. E. coli produces the enzyme β-galactosidase to digest the lactose into glucose and galactose. However‚ it would be inefficient to produce enzymes when there is no lactose available‚ or if there is a more readily-available energy source available such as glucose. Therefore there must be something controlling the expression of this enzyme. The purpose of the experiment is to determine whether
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attachment of bacteria to such a surface correlates with the surface zeta potential of the nanoparticles. All studied Ag-PIII surfaces reduced the proliferation of both types of bacteria studied (Gram-positive Staphylococcus aureus and Gram-negative Escherichia
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Two different types of procedures were carried out over the course of this experiment: tube inoculation and plate inoculation. We started with plate preparation. Three different lysogeny broth‚ or LB‚ agar plates were prepared for E. coli growth every other week: a control where water was used‚ one for triclosan‚ and one for streptomycin. Using an inoculation loop‚ E. coli was transferred from the test tube to the agar plate. This was done to each plate twice‚ creating a grid-like pattern of bacteria
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