TITLE Lab #4 Soil –vs- Water Microbiology INTRODUCTION There are 4 learning goals for this lab: 1. Collect samples and compile data from at least 2 sources (soil; water) of your choice. 2. Identify at least 2 Prokaryotes (bacteria) and 2 Eukaryotes (fungus) in your samples. 3. Research the importance of bacteria and fungus to Earth in a non-medical context. 4. Compile a high-quality‚ 3+ resource (excluding the lab worksheet)‚ MLA-cited in-text laboratory report using the scientific method
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BACTERIAL TRANSFORMATIONS USING PVIB II. INTRODUCTION Transformation is the manipulation of a bacterial cell’s DNA in order to alter the cell’s genotype or phenotype by absorbing free DNA from its surroundings. In this lab‚ pVIB plasmid will be used. A plasmid is a segment of DNA that can incorporate itself into the bacterial DNA. Although is not required for growth of the bacterial cell‚ plasmids can provide advantages in stressful environments such as the ability to adapt as environmental
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In the business capital of India‚ There is a country named Mumbai‚ formerly known as Bombay. Was recently a headliner for high increase in water contamination in due to mismanagement of BMC. BMC- Bombay Municipal corporation‚ is a corporation of the water and sewage systems dealing within the city. Water crisis in Mumbai is beyond unbearable‚ 19% of water consumed is contaminated with sewage water‚ E. Coli‚ sand particles‚ and other harmful bacterial content besides other foreign particles. "According
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Documentary Analysis: Food Inc. Ever wondered where the food you eat came from? The documentary Food Inc. does exactly that. This film is made by Robert Kenner and Eric Scholosser about the food industry here in America. It focuses on the food industry being a few giant corporations who control everything we eat. The main argument the film brings is that the food industry is very immoral and the government is not doing everything they can to maintain quality‚ which is putting the public safety
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Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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There were eight antibiotics used for this experiment; chloramphenicol (C)‚ erythromycin (E)‚ fusidic acid (FC)‚ oxacillin (OX)‚ novobiocin (NO)‚ penicillin G (PG)‚ streptomycin (S)‚ and tetracycline (T). The mode of action of Chloramphenicol is the inhibition of protein synthesis. Chloramphenicol irreversibly binds to a receptor sit on the 50S subunit of the ribosome on the bacteria that inhibits the enzyme peptidyl transferase. This inhibition results to the prevention of the transfer of amino
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BIMM 101: Recombinant DNA Lab Lab 7 & 8 Assignment Part B 1. A genomic library is a “collection of recombinant vectors or clones‚ among which is representative of the entire genome of the organism” (BIMM 101 Lab Manual‚ 47). In order to create a genomic library‚ genomic DNA from Vibrio fischeri was first isolated then treated with Sal I restriction enzyme to generate inserts (smaller fragments of DNA). Sal I restriction enzyme was also used to treat the vector plasmid in order to digest
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Introduction Water is necessary to life but it can be unhealthy too. In general‚ the water quality is poor in developing countries like Kenya. Water is a vector of diseases like yellow fever‚ diarrhea… Water purification is very important in these countries but people don’t have a lot of time to spare‚ they work or they are in the field and in general women must spend a significant amount of time walking to collect water. So we need to find a quick‚ easy‚ and economic solution that is as simple
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Observation of Bacteria on Chicken and Handrail and the Effect of GermX® and Alcohol on Them. Sujit Bhimireddy‚ Daryl Onwuchuruba‚ Naveen Sood‚ and Manasa Tripuraneni Biology 106 Abstract Bacteria are found everywhere on this planet. This experiment is aimed at observing some of the bacteria that surround us in places that we use everyday. We will look at bacteria found on a piece of chicken and bacteria found on a handrail. The effectiveness of antibacterial substances such as GermX® and
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When I received my slant culture‚ I immediately made a streak plate and started the Gram Stain protocol1. In the first two slides‚ I made and stained‚ my results were ambiguous. Without putting the slide into the microscope‚ I noticed one was pink and the other purple. To resolve it‚ I discarded my slides and started again and was extremely careful in proceeding the Gram Stain. I concluded that my unknown A was gram-negative bacilli. Using the dichotomous key for gram-negative bacilli I started
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