The spot inoculation method was followed for antifungal activity of bacterial strain Paenibacillus elgii TS33 25. The nutrient agar medium was prepared‚ sterilized at 121ºC‚ poured into the sterilized peteriplates and allowed to solidify under aseptic conditions. After solidification bacterial strain Paenibacillus elgii TS33 was spot inoculated on nutrient agar medium and incubated at 37ºC for 48 hours. After incubation the molten potato dextrose agar medium containing the spores of test fungus‚
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Staphylococcus aureus‚ otherwise known as Staph‚ is a bacterium that has naturally acquired resistance to many current antibiotics. Specifically‚ the MRSA (methicillin-resistant Staphylococcus aureus) strain has acquired resistance to methicillin. Staph frequently colonizes in human nasal passages‚ which serve as reservoirs for transmission. The following texts focus on Staph’s nasal colonization and mode of transmission‚ inspired by the research of Dr. Alexander Cole at UCF’s Burnett School of Biomedical
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Collaboration is defined by working in a group of people to reach for that goal. When working in a group of people you come up with things for your experiment as in what procedure you’re creating. During your collaborations you discover things that happen as a whole instead of as an individual. An example of collaboration was during biology we came up with ideas to create a procedure for the gummy bear lab as a class to get the same data Communications is defined by talking with others about the
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Symptoms and Causes: Salmonella enterica serovar Typhimurium‚ also known as S. typhimurium‚ is a Gram-negative‚ rod-shaped‚ flagellated‚ facultative anaerobe microorganism. S. typhimurium can be found in animal feces and raw contaminated foods such as poultry‚ beefs‚ sea foods‚ tainted fruits and vegetables (3‚ 5). Some of the vectors of S .typhimurium are birds‚ reptiles‚ and aquatic vertebrates (5). After the consumption of the bacterium‚ S. typhimurium is found living and growing in the
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The identification of an unknown bacterial environmental isolate through a series of morphological‚ physiological and differential exercises. The purpose of the environmental isolate report is to learn what is necessary in order to take an unknown environmental isolate (EI) and identify it. This was achieved through a series of exercises that provided information on the morphological‚ physiological and biochemical traits of the EI which were then compiled and interpreted in order to make a presumptive
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Gram negative bacteria resistant patterns are troublesome to the general public because common illnesses could suddenly become deadly. Carbapenem antibiotics are seen as a last resort. If gram-negative bacteria develop a resistance to this antibiotic‚ antibiotics could become useless in treating a super resistant strain of gram-negative bacteria. NMD-1 is one of the most resistant genes found in gram negative bacteria. If this genes is readily passed between different types of bacteria‚ widespread
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1. Antioxidant Compounds in Red Beet Excessive formation of free radicals such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) occur as a byproduct of cellular metabolism. Free radical generation causes oxidative stress which has been associated with undesirable health effects (Valko et al.‚ 2007). Antioxidants are needed when ROS are generated inside the body‚ as the first line of defense (Lobo et al.‚ 2010). Halliwell & Gutteridge (2015) describe an antioxidant as “any substance
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Student Lab Section 6 E. Coli Genetic Transformation with pGLO Plasmid Introduction: Genetic transformation is where one organism takes on a characteristic from another organism (Bacterial Transformation 2013). For this experiment we used the bacteria E. Coli to take in foreign jellyfish DNA which will allow it to change genetic material. This experiment determines the effects that the plasmid pGLO has in transferring the Green Florescent Protein found in a jellyfish into the bacteria. It
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Jennifer Hauss March 4‚ 2015 Bacterial Transformation Lab Report Introduction In this lab‚ the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. One plate was the control plate‚ having only the LB or agar for the bacteria and negative pGLO‚ which is the liquid not containing the plasmid. This is the plate that was compared with the three
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Foodborne Illness Week 4 Laura Shepherd SCI/163 November 26‚ 2012 Raymond Kahler Foodborne Illness Week 4 Clostridium perfringens One of the most common foodborne illnesses in the United States is Clostridium perfringens (C.perfringens). According to the Center for Disease Control‚ it is responsible for approximately one million cases of foodborne illness each year (Center for Disease Control‚ 2011). This bacterium is an anaerobic gram-positive bacterium. This means that it requires little
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