MANIPULATION OF BACTERIA INTRODUCTION: In this experiment that we performed‚ there were many methods that were used to help us manipulate and identify the bacteria E.coli on a MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is
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Describe the properties and uses of plasmids in G.M. experimentation. Plasmids are extrachromosomal genetic elements found in a variety of bacterial species. They are double stranded; autonomously replicating‚ supercoiled‚ covalently closed circular (ccc) DNA molecules that range in size from 1 kb to greater than 200 kb. Often‚ plasmids contain genes coding for enzymes that‚ under certain circumstances‚ are advantageous to the bacterial host (Table 1). Table 1. Some of the phenotypes conferred
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Individual Case Analysis Companies in Crisis- What to do when it all goes wrong? Discussion Questions: 1. What should the company do regarding the following specific issues? a. Should the company recall all of its fresh apple juice‚ just those batches considered potentially contaminated‚ or not recall any of the product? I think Odwalla should recall all of its fresh apple juice in order to prevent consumers from having to worry about purchasing the product and getting contaminated
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The Phenomenon that is Genetic Transformation Matt Kimmel November 17‚ 2011 Bio 121-012 Introduction Genetic transformation is when the genetic makeup of an organism is altered by it receiving external genetic material (Barnhart and Hopper‚ 2011). Bacterial transformation was first seen during an experiment by Fredric Griffith in 1928. In the experiment there were two strains of bacteria‚ a virulent strain‚ and non-virulent strain. Virulent simply means disease causing‚ and therefore non-virulent
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Name one disease/condition that could be caused by your organism‚ at least two of its symptoms and what treatment is most often utilized (Antibiotics must be named). (Infection does not count‚ you must be more specific). Diseases can be human or animal. If no disease is found‚ list and describe one industrial use. Industrial use should include any products it being used to make/produce and if the organism is genetically altered for that use. Your answer to this question should be in complete sentences
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DATE: November 4‚ 1996 TO: Stephen Williamson‚ President and Chief Operating FROM: Monica Pearlman 21 Bascom Drive East 101 SUBJECT: Addressing Contamination linked to Odwalla Juice Products ------------------------------------------------------------------------------------------------------------------------------------------ Odwalla‚ Inc.‚ a health-conscious juice producer‚ must challenge effects that its apple juice product is contaminated and has been linked to the causes of several
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Odwalla Case Assignment In the Odwalla Case there are many stakeholders. There are the employees‚ the customers‚ the communities‚ the media‚ the suppliers‚ and the shareholders. The most important stakeholder in this case is the owners: founder‚ Greg Steltenpohl and CEO Stephen Williamson. I know they are the stakeholders because they not only have a direct economic transaction with the company but also‚ their actions affect the outcomes of the business. For example‚ they set up the values of the
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Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
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I. Soil-Transmitted Helminthiasis Parasitism is a symbiotic relationship in which one organism‚ the parasite‚ benefits at the expense of the other‚ the host. Soil-transmitted helminthiasis is a human infection of the intestinal tract caused by different species of parasitic worms acquired through contact with contaminated soil (Bethony et al. 2006). Light helminth infections are well endured by human hosts and usually have no symptoms‚ but some serious cases cause morbidity and mortality (CDC‚ 2013;
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