The Effect of Varying Ligation Conditions on Ligation Efficiency Introduction The goal of this experiment was to successfully ligate RFP into P1 and transform E. coli with P1-RFP plasmid‚ which contains the P1 promoter‚ RBS‚ and RFP gene. We tested several different ligation conditions and assessed how they each affected ligation efficiency. The two variables that we tested were: molar ratio of insert (RFP): vector (P1 plasmid)‚ as well as the effect of removing the stuffer from the digested plasmid
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This semester while we worked through this unit‚ we were trying to figure out why Addie got so sick and how we could look at the juncos as a comparison study. We learned about the relationship that antibiotics and bacteria have by looking at our NetLogo Simulation and Petri Dish Lab. Also‚ we learned about various CDC recommendations for using antibiotics and preventing bacterial illnesses. When we looked at the juncos‚ we were able to learn how common ancestry works within any species. Throughout
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31 October 2011 Identification and observations of Citrobacter Freundii Introduction Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections‚ diarrhea‚ and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly‚ it is
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ABSTRACT In todays world of ever evolving genetic mutations the incidence of drug resistant forms of microorganisms are on the rise. The importance of identifying these pathogens and their related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine
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Miguel Felix pGLO Transformation Mr. Betz AP Biology 14 December 2012 Abstract The purpose of this experiment is to determine the effects of the addition of a plasmid to a bacterial cell. The bacteria E. Coli was separated into two groups: one where the pGLO plasmid was added to the bacteria‚ which contains the genes of fluorescence and resistance to antibiotics‚ and the other lacking the plasmid. The two groups then placed in agar plates simulating different environments: the bacteria lacking
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Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab‚ the Green Fluorescent Protein‚ which is typically found in the bioluminescent jellyfish Aequorea
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Eldon Roth‚ the CEO of Beef Products Inc has been producing Lean Fine-Textured Beef through a process he invented at least 20 years ago. The FDA and the USDA has stated that this product is completely safe. The product has not been attributed to a single illnesses or death. This fact is impressive given that ground beef is especially susceptible to E. coli contamination. According to the Centers for Disease Control and Prevention‚ E. coli causes an estimated 73‚000 illnesses and 61 deaths a year
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In the world‚ some people see bacteria as something that is harmful and that causes nothing but illness and infections. Bacteria can actually be helpful as well. It can provide vitamins to your body‚ help digestion‚ destroy bad organisms‚ help make medicines and also help out with the environment. In this research paper‚ it will describe two bacteria that are helpful and sometimes harmful to humans and the environment‚ which are E. coli and Lactobacillus Acidophilus. E. coli E. coli is the bacteria
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Hogs can get many diseases‚ you need to know the causes‚ symptoms and treatment‚ also prevention. There are a large number of diseases that can affect a hog and this will vary from place to place depending on the area you where you live and raise hogs. Remember that so many diseases are entirely preventable as you need to keep the feed lots and houses sanitary‚ unclean and filthy lots and houses will encourage disease. African Swine Fever- This is likened to hog cholera and is the most devastating
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My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown‚ we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture‚ therefore we are very confident that it is correctly identified. We identified that K. pneumoniae
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