In the experiment involving varying pH levels‚ E. faecalis‚ S. epidermidis‚ L. lactis‚ and L. casei were subjected to different pH levels and then were allowed to incubate in order to determine the minimum‚ maximum‚ and optimum pH levels for growth of specific bacterial species. It was found that lower pH levels between 2 and 4 inhibited or promoted little to no growth for E. faecalis‚ S. epidermidis‚ and L. casei. It is evident that these bacteria were resistant to alkaline environments‚ however
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Lab Practicum‚ Micro 200‚ study guide When you did your hand wash experiment‚ you decided a variable to test‚ say‚ hot vs cold water‚ you called this variable an “experimental variable” since it was the variable you were testing experimentally. In order to get meaningful data‚ you need to keep other things the same‚ for example the washing time had to be the same for both‚ you called the washing time a _Control_______ variable. Through the semester‚ you have used two bacteria that produce
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Unit 7 Introduction In this piece of coursework‚ there are few amounts of ideas and experiments that I could achieved of which different products to test for my concluding idea. The type of bacteria that I am going to discuss and chosen is E-coli. I will also going to research the effectiveness of antibacterial cleaning products‚ for instance sanitizer. I will also‚ research which is the most effective product for the house hold and some other work places. Background Information What are
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The experiment consists of 5 steps—Inoculation‚ fermentation‚ cell lysis‚ inclusion body solubilization‚ and peptide purification. In the first two steps‚ E coli with Snake 6 peptides is cultivated. Ultraviolet–visible spectroscopy (UV-vis) is used to quantify bacteria through detection of their optical density (O.D.). After reaching a certain concentration in the fermentation batch‚ inclusion bodies (IBs) are extracted from these E coli by adding Bugbuster lysis solution and lysozyme. Polyacrylamide
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1. The overall goal of this lab was to carry out experiments that clearly demonstrate the different ways DNA genetic information‚ specifically transduction and conjugation. The first half of this experiment focused on exploring the mechanisms of transduction. This was done by creating a spot titer plate for phage carrying kanamycin resistance and E. coli. E. coli was then proven to have gained kanamycin resistance throughout transduction as demonstrated by its ability to grow on a medium containing
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To purify the PCR amplicon‚ both digested wt-goi and mut-goi from other components. A purified digested gene product is more advantageous for the DNA ligation‚ in which the gene products with sticky ends will be inserted to a plasmid vector. Also‚ to transform E.coli DH5α cells by introducing the plasmids DNA which contains the gene of interests into the E.coli strain(DH5α). The plasmid DNA can replicate inside the transformed E.coli DH5α cells‚ only successful transformed cells can produce the protein
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BSPT 13 Ms. Mae Maghari Escherichia coli is a Gram-negative bacillus that is found in the GI tract of all humans. The strains and sero types of E. coli that are part of the indigenous microflora of the GI tract are opportunistic pathogens. They usually cause no harm while in the GI tract‚ but have the potential to cause serious infections if they gain access to the bloodstream‚ the urinary bladder or a wound. Escherichia coli‚ also known as E. coli is a bacterium that
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Yearin Kwon 2nd Period 12. 01.2012 Research Paper E Coli The Escherichia Coli 0157:H7 or also know as E Coli‚ is a food born pathogen that can cause serious illness to humans at any age. The E Coli can also lead to death if left untreated. There are many symptoms with the E Coli. And one of the most obvious symptoms is having a bloody diarrhea (Not all forms of E Coli have the same symptoms). The symptoms can be easily confused with other disease since it is such a common symptom. The
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Lab12-Medical Microbiology- Part1– Differential Media/Biochemical Tests‚ Sp2012 (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs. There are several sections to this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read
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Amanda Lab Report 1 Microbiology Laboratory Title – Differentiating Organisms using the Gram Stain Introduction The experiment conducted was based upon the known attributes of two different groups of bacteria‚ those that are gram positive‚ and those that are gram negative. Using a specific staining procedure‚ it is possible to differentiate the two types under a microscope The gram stain method of differentiation is possible because of differences in the cell membrane between the two categories
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