OBJECTIVE The objective of this lab was to determine the effects of two unknown drugs on the microtubule pathways in COS-7 cells through the process of immunostaining. METHOD Before arrival‚ the COS-7 cells were grown in culture dishes submerged in a growth medium that contained bovine serum. Two coverslips covered in the COS-7 cells were provided. After washing the coverslip with Phosphate Buffer Saline (PBS)‚ one coverslip was treated with the control solution‚ and the other was treated with
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This experiment tested the growth of E.coli with inserted plasmid on an agar plate with Ampicillin. One colony of E.coli resistant to Ampicillin was grown during this experiment. The overall goal of the experiment was to successfully grow E.coli on the agar plate‚ which would show that the plasmid had been effectively inserted into the bacteria’s genes. This experiment helped students understand how plasmids were inserted into bacteria and used in real life situations. It also showed how the bacteria’s
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In this lab experiment‚ we compared the amount of germinated Raphanus sativus’ in normal and frigid temperatures. My group and I predicted that more seeds in the normal temperature atmosphere would germinate‚ rather than the seeds in the frigid condition. The experiment starts with two petri dishes‚ and we filled each dish with fifty radish seeds. We made our first observations about the seeds that we see. We labeled one petri dish “Control‚” and the other was labeled “Experimental.” The experimental
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Intro to Microbiology: Gram stain: Gram + Gram - Exotoxin Except Listeria has endotoxin Endotoxin Except Capsules Strep pneumo VERY Dangerous: (Some Strange Killers Have Pretty Nice Capsules) 1. Salmonella 2. Strep 3. Kleibsiella 4. Hemophilus B 5. Pseudomonas 6. Nisseria-(largest capsule- most likely cause of any toxicity) 7. Citroacter Gram (+) Gram (-) N-acetyl muramic acid (Nam) + N-acetyl glucosamine (Nag) = Peptidoglycan wall Contains
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Your Full Name: UMUC Biology 102/103 Lab 1: Introduction to Science INSTRUCTIONS: On your own and without assistance‚ complete this Lab 1 Answer Sheet electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus). To conduct your laboratory exercises‚ use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering
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The debate of whether bacteriophages could replace antibiotics is one of the many health issues to be explored in this essay. Bacteriophages are viruses that specifically target bacteria in your body to destroy it. Many scientists believe that bacteriophages will replace antibiotics in the fight against bacterial/diseases‚ although ideal‚ scientist do not know the long term effects of using this mechanism of defense. More research is needed and thus the push for bacteriophage usage may be reluctant
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Nicoleta Nedelcu Caffeine-induced effects on heart rate in zebrafish embryos and possible mechanisms of action Observation Caffeine is found in coffee‚ tea leaves‚ cocoa beans‚ and kola nuts. It is used in prescription and over the counter drugs. It increases blood pressure‚ mental alertness‚ and gastrointestinal motility. Cardiovascular development with caffeine and other small molecules can be studied relatively easy using zebrafish. The cardiovascular system is complete in two days after fertilization
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2. Steps for Southern Blotting Digest the DNA with an appropriate restriction enzyme. Run the digest on an agarose gel. Denature the DNA‚ which would separate double-stranded DNA into single-stranded DNA. Transfer the denatured DNA to the membrane. Probe the membrane. Visualize your radioactively labeled target sequence. If you used a radiolabeled 32P probe‚ then you would visualize by autoradiograph. 3. a) She placed the nucleotide as she did because the EcoR I cuts in one
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“DNA Fingerprinting” Homework Assignment (10 points) Question 1 A schematic of the vector p7012 is shown. The restriction enzymes listed cut only where indicated; they do not cut anywhere else in the vector or insert. a) A schematic of gene W is below. You want to clone all of gene W DNA into the p7012 vector. Give three different strategies that you could use to clone gene W into p7012‚ and obtain colonies that contain a recombinant plasmid. * Strategy 1 uses the restriction enzyme(s)
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Toxic Shock Syndrome (T.S.S.) You may have never heard of Toxic Shock Syndrome or T.S.S. It may help to know what T.S.S. is and its causes‚ how to prevent it‚ and how to treat it. Toxic Shock Syndrome is form of blood poisoning caused by overgrowth of Staphylococcus aureus or Streptococcus bacteria. Sometimes these strains of bacteria cause a toxin TSST-1. Its causes have been linked to use of tampons but it can also be caused by surgery and skin wounds. T.S.S. symptoms include a high
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