NA transformation of E. coli: The two plasmids were added to individual tubes containing E. coli and one with no plasmids. The three samples of E. coli were heated in a 42°C water bath for 90 seconds to heat shock the bacteria so that the plasmids would be taken up by the E. coli. These samples were then incubated at 30°C for half an hour and then plated on LB agar. Each tube was plated on an LB plate and a LB + ampicillin plate. Ampicillin is an antibiotic that is effective against E. coli‚ both
Premium Escherichia coli Bacteria DNA
1) TITLE (2pt): Testing antimicrobial props in soaps 2) INTRODUCTION (6 pts): In this experiment we wanted to test how effective different types of soaps against E. Coli. For this experiment we were interested in looking at antimicrobial soaps vs. non-antibiotics soaps in their effectiveness to kill E. Coli. The protocol that we used was the Kirby Bauer technique. This technique was used to test the antimicrobial properties of each soaps. (“Kirby-Bauer” 2015). 3) METHODS (6 pts): We first started
Premium Bacteria Escherichia coli Antibiotic resistance
The purpose of this experiment was understand the process of transformation and the effects on gene expression. The pGLO(-) culture had growth on the LB medium‚ while the LB amp and LB amp + ara mediums had no growth. It was expected that the LB medium had growth on the plate because it served as a control. The LB amp and LB amp + ara had no growth or glow under UV light because they were not successfully transformed and still contained the antibiotic ampicillin that prevented the growth of E. coli
Premium Escherichia coli Bacteria DNA
Lux G experiment is performed at the University of California San Diego on May 21st until June 4th of 2018 by Gloria Karem. This experiment is based on the purification of LuxG by using multiple purification and lysing methods: Brad Ford Assay‚ SDS-PAGE‚ affinity purification‚ lysozyme treatment‚ nickel column‚ sonication‚ and flavin reeducate activity assay. His-tagged protein is expressed in E. coli from pGhis‚ with the T7 RNA lac repressor induction system.5 Sonication is used‚ it’s why bacteria
Premium Bacteria Escherichia coli DNA
Biol 228 Virulence factors of Legionella pneumophila The Gram-negative bacterium Legionella pneumophila contains a singular monopolar flagellum which is composed of a major subunit‚ the FlaA protein. Motility is associated with the infectious phase of L. pneumophila. In the initial phase‚ the replicative phase‚ the bacteria are immotile and have nonexistent or low toxicity. The growth of flagella leads to the infectious phase‚ where the new motile form of L. pneumophila is highly toxic and
Premium Secretion Microbiology Cell
PRODUCT FIT Fruit and Vegetable Wash PRODUCT LOGO [pic] ABOUT THE PRODUCT Fit Antibacterial Fruit & Vegetable Wash kills 99.9% of harmful bacteria -- like E. Coli‚ Salmonella sp.‚ Staphylococcus aureus‚ and Listeria – that have been associated with food-borne illness‚ and is only for use on cut chopped‚ sliced and other processed produce. Fit is 100% Natural‚ and unlike chlorine or other harsh chemicals‚ is completely safe to use. Fit rinses away clean‚ leaving no aftertaste or smell…just
Premium Vegetable Nutrition Escherichia coli
DATE: September 4‚ 2008 TO: Stephen Williamson‚ President and Chief Operating Officer‚ Odwalla‚ Inc. SUBJECT: Addressing Contaminated Juice Products ----------------------------------------------------------------------------------------------------------- Odwalla‚ Inc.‚ a health-conscious juice producer‚ must confront implications that its apple juice product is contaminated and has possibly caused several outbreaks of the E. coli virus. It is a truly unfortunate situation when
Premium Brand Management Escherichia coli
Antimicrobial Properties of Plants A plant with antimicrobial properties means that it destroys or inhibits the growth of bacteria and fungi. Aim: our aim was to see weather or not garlic‚ mint and grass contained antimicrobial properties and if they did how effective they were we were trying to see weather the bacteria will grow around the different plants. Control Test: As a control test what we did was‚ we dipped a paper disc into the alcohol solution we used to dissolve the plants; the
Premium Bacteria Microbiology Microorganism
Shigella Introduction: Shigella is a very infectious bacteria that causes Shigellosis. I. Morphology of Shigella • Gram-negative • Nonmotile bacillus II. 4 different species of Shigella • S. dysenteriae o Most severe type of shigellosis because it secretes Shiga Toxin Shiga toxin- stops protein synthesis in host’s cell. • S. flexneri o Is the predominate species in developing countries. • S. boydii • S. sonnei o Is the most common species isolated in industrialized nations.
Premium Bacteria Protein Microbiology
Proteus Mirabilis Proteus mirabilis is part of the Enterobacteriaceae family. It is a small gram-negative Rod‚ and a facultative anaerobe‚ lastly‚ it is a Prokaryote. Furthermore‚ Proteus mirabilis is characterized by its swarming motility‚ its ability to ferment maltose‚ and its inability to ferment lactose. P. mirabilis has the ability to elongate itself and secrete a polysaccharide when in contact with solid surfaces‚ making it extremely motile on items such as medical equipment (catheters)
Free Urine Urinary tract infection Kidney