Introduction to Microbiology – Case Study 1 Kristen Alejos You are working as an emergency room nurse in Topeka when a mother brings in her 8 year old son because of severe‚ bloody diarrhea. When questioned‚ the boy said that it hurts when he goes to the bathroom and his mother said he had vomited in the previous two days. When asked about their previous movements‚ the mother and son both mentioned a backyard barbeque that had taken place 6 days earlier. The boy complained that his mother
Premium Escherichia coli Bacteria Microbiology
1- Synthesis of silver nanoparticles by Bacillus sp.: The microorganisms in the most contaminated toilet Sink were isolated by dilution technique. The dominant bacterial strains are identified as Bacillus sp.‚ on the basis of morphological and biochemical characteristics. Gram’s staining: Gram positive and rod shape. They were serially diluted and spread on nutrient agar plates. The starch has been hydrolyzed and a clear zone by the addition of iodine solution. Visual identification: When bacterial
Premium Bacteria Microbiology Staining
MATERIAL AND METHODS Recombinant protein production The recombinant T-Rp3 was expressed in E. coli BL21 (DE3) as previously described (Favaro et al.‚ 2014) and purified by a single step His-based affinity chromatography in an ÄKTA purifier FPLC (GE Healthcare). Protein was then dialyzed against 50 mM MES buffer (pH 6‚0) overnight at room temperature‚ frozen in liquid nitrogen and stored at -80 ˚C after 0.22 μm pore membrane filtration. Evaluation of DNA-protein interaction by gel retardation
Premium Bacteria DNA Molecular biology
OBJECTIVE The purpose of this lab was to troubleshoot causes related to PCR components and develop an experiment that would test if the Taq amount is suitable for the PCR reaction to run correctly. INTRODUCTION Thermus aquaticus (Taq) DNA Polymerase is a bacterium that lives in thermophilic conditions and has been identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR (Noronha & Mullins‚ 1992). Why might you not be getting any bands on your
Premium DNA Bacteria Gene
The antifeedant activity of the extracts choice and no-choice test methods‚ after 24 and 48 hours in each test‚ against 7 day old (3rd instar) larvae of S. litura on castor leaves. Stock solutions of the test compounds were prepared in the carrier solvents. Further dilution was done in emulsified water by maintaining emulsifier (Tween – 80) level at 0.5 % to yield various concentrations. Castor leaf discs (4 cm diameter) were punched out from the washed and dried castor leaves. Leaf discs were dipped
Premium Bacteria Microbiology Escherichia coli
Application of Epidemiology – A Case Study Analyze Good Health Hospital s records and itemize records and itemize recent nosocomial infections that occurred within the past year. In your report‚ categorize the different parameters (i.e.‚ person‚ time‚ place‚ ethnicity‚ and gender) used in the compilation of data into the information summative. An outbreak of E-coli has occurred in Good Health Hospital‚ this has become a major problem since the outbreak occurred in Ward
Premium Infectious disease Epidemiology Infection
The problem that was required to be investigated in this laboratory experiment was to determine which type of cleaning product would be able to prevent the most bacterial growth when placed in a neutral environment. In order to answer this problem‚ 8 different disinfectants were tested‚ including sterile water‚ and their after two days their zones of inhibition were measured and recorded. A hypothesis that was formulated before experimenting was‚ “If the type of disinfectant is Mouthwash‚ then it
Premium Bacteria Microbiology Water
Levishon and kleven (2000) discussed that the most dramatic disease of M. gallisepticum was chronic respiratory disease in meat type birds‚ often as one of several aetiological agents in a multi-factorial disease complex. Phenotypic variation of major surface antigens occurs at high frequency which was a probable explanation for chronic infection by M. gallisepticum despite a strong immune response. Bradbury et al. (2001) mentioned that avian Mycoplasma was caused by several pathogenic Mycoplasmas
Premium Bacteria Immune system Infectious disease
Clostridium tetani is a bacteria which can be found in soil and faeces‚ and causes the disease Tetanus. [Human Illnesses]. Clostridium tetani bacteria reproduce by binary fission because they are a bacteria. [St Margaret’s College]. In binary fission‚ bacteria reproduce by splitting themselves in half on a regular basis. [St Margaret’s College]. In ideal conditions‚ the Clostridium tetani bacteria may reproduce by binary fission every 20 minutes. [St Margaret’s College]. To live and reproduce by
Premium Bacteria Immune system Infection
Title: E. Coli Transformation with a Plasmid DNA Containing the GFP Gene Introduction: Bacterial transformation is the process of bacteria taking in and expressing exogenous DNA. This has led to many other discoveries. In order for bacterial transformation to occur the bacteria must be in a certain physical state to be able to take in DNA. This is called competency and it allows the cell membrane to be permeable so DNA can pass through. Currently researchers are studying the transformation
Premium DNA Plasmid Gene expression