ASSAYING PLANT SUBSTANCES FOR ANTIBACTERIAL ACTIVITY PURPOSE To test plant extracts to see if they possess antibacterial activity. PROCEDURE First create a list of plant extracts being tested and choose 2 extracts per group. The plants being tested are Oregano‚ Tea tree‚ Eucalyptus and Colloidal Silver. Obtain two nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of
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In the documentary Food Inc.‚ food-borne illness was one of the leading themes and also gave a story to go along with it. The director’s main argument was to show consumers where their food is coming from and the beginning stage of how it all begins‚ before getting on the table. “The Center for Disease Control estimated that each year roughly 1 in 6 Americans get sick from foodborne diseases” (CDC‚ 2016). There are many types of food-borne illnesses‚ but the one thing that they all have in common
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Dannon Company has a trademark of Activia in its products. One of its products is the Light and Fit yoghurt. The light and fit comes in variety of flavors‚ raspberry‚ banana and strawberry just to name a few‚ with no artificial color and a natural touch. This product is advertised to be multi- functional with its great works with women’s gastrointestinal hygiene and beauty. The product is said to bring about what people want from any food supply. The company is mostly targeting young ladies‚ middle
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INTRODUCTION The Gram Negative lab had two parts; the aim of the first part is to determine the concentration of gram negative bacteria in a water sample collected from a creek near Providence Road‚ Strickling. Gram negative bacteria have a cytoplasmic membrane‚ a thin peptidoglycan layer‚ and an additional outer membrane composed of phospholipids and lipopolysaccharides. Because gram negative bacteria have a relatively thin cell wall when compared to gram positive organisms‚ they are consequently
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Bio Lab Report Title: Gram-Negative and Gram-Positive contamination in yogurt. Introduction: There is bacteria that is beneficial and harmful in the human. Yogurt was choosed to be the best example that contains this bacteria. What happens to be the difference between these two types of bacteria‚ and how are they harmful? The bacteria is considered “Gram-Negative‚” and the substance is considered contaminated when there is more negative bacteria than positive bacteria. This will prove or disapprove
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Key words: Clostridium perfringens‚ diarrhea‚ dog‚ hemorrhagic gastroenteritis. Introduction Enteric clostridial infection in dogs are life-threatening condition usually caused by Clostridium perfringens and Clostridium difficile. The pathogenesis of enteric clostridial infection requires two factors; the presence of these microorganisms in the intestine and a change in the enteric microenvironment. C. perfringens is a Gram-positive‚ spore-forming‚ obligately anaerobic‚ non-motile bacillus and
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The Expression and Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained
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Mini-Lab Report Objectives: There were two major goals of the “Observing Bacteria and Blood” experiment. The first was to establish a familiarity with the proper techniques for using a microscope. The experiment focused on both the basic components of a microscope (i.e. how to adjust the knobs and levers for the desired result) as well as how do decide the proper objective to use for observing a specimen. The second goal major goal of this experiment was to practice observing live specimens
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Lab 6: Isolation of Chromosomal DNA Mic 428L/ Section 001 Introduction: In biological research to address and eventually answer a multitude of questions‚ usually involves isolating chromosomal DNA. The purpose in this particular lab was to isolate chromosomal DNA from mutants grown and observed in lab 5 and then digest the DNA using a restriction enzyme. The fragments left from digestion will be ligated and then transformed into a strain of E. Coli DH5αλpir containing the pir gene pi product
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In 2015 Chipotle Mexican Grill faced an E. coli epidemic in many of its establishments across the country. The fast-food chain has grown rapidly in a short time‚ but the recent bacteria scare is putting strain on Chipotle’s sales. Back in October 2015‚ multiple cases of people getting sick were reported in the states of Washington and Oregon. As of January 2016‚ 55 people had fallen ill across 11 states. Chipotle’s biggest problem is that it had no idea what products were contaminated with E. coli
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