Title of the lab: Transformation : Bacterial Genetics Purpose of the lab: The pupose of the lab was to transfor a bacterial E. Coli by using the green flurescent protein from the jellyfish. Another important that was fferdone by making the cell competency‚ meaning that it will be able to take on additional DNA. This was done when the plasma was added. Materials: 1. 37 o C water bath 2. Ice 3. Sterile transfer pipette 4. Foam tube rack 5. Transformation solution (CaCl2) 6. pGLO plasmid 7. Sterile
Premium Bacteria Escherichia coli DNA
Objective: The main objective for this lab was to use the Bradford and DC Protein Assay in order to determine our concentration of E. Coli BL21(DE3) lysate. Principle of Methods: To accomplish this objective‚ each pair of lab partners received an E. Coli lysate sample which we had to thaw by warming it with our hands and centrifuge it. We extracted a total of 12 samples with known concentrations of bovine serum albumin and 10 samples of different amounts of lysate put into them. Using the different
Premium Escherichia coli Bacteria DNA
2.3.2 Counter Ion “Most bacterial cell walls are charged with a negative charge‚ so most antibacterial polymers must be charged with a positive charge to facilitate the adsorption process. The composition of the counter ion‚ or polymer-linked ions‚ is used to balance the charge‚ it also affects the antibacterial activity. The counter ion has positive charge‚ which is a strong ion-pair with the polymer will inhibit the antibacterial activity because it will prevent the polymer from interacting with
Premium Bacteria Penicillin Antibiotic resistance
RESULTS AND DISCUSSION E. coli and S. aureus were tested to determine effectiveness of photodynamic inactivation with the use of methylene blue as photosensitizer and blue LED as alternative light source. Table 1 shows the resulting number of colonies for each replicate of every treatment per bacteria tested. Each of the treatments was averaged to get the mean number of colonies per treatment. All experimental setups were produced in quintuplicate‚ however only three of the five replicates were
Premium Bacteria Escherichia coli Antibiotic resistance
The experiment was separated into four stages‚ the first being preparation of cell competency. In this stage two vials were placed in ice baths‚ one vial containing 50 µL of E. coli and the other containing a CaCl2 solution. 630 µL of the CaCl2 solution was then transferred to the E. coli vial‚ using a sterile pipet. After tapping the tube to mix the solution‚ it was then returned to the ice bath to continue incubation for at least 10 minutes. The cell competency preparation was carried out by the
Premium Escherichia coli Bacteria DNA
Rita Bernstein On November 14‚ 2012‚ Rita Bernstein visited Bergen Community College to talk about Escherichia Coli O157:H7. Escherichia coli or better known as E.coli is a tube-shaped bacterium that is found in the stomachs of warm-blooded animals. O157:H7 is a member of the E.coli family‚ which produces shiga toxin‚ and are capable of producing bloody diarrhea. E.coli O157:H7 attacks red blood cells‚ it interrupts flow of oxygen‚ and it breaks down walls of vessels. E.coli can
Premium Escherichia coli Bacteria
estimates indicate that one in six or 48 million people are stricken with a foodborne illness each year‚ resulting in 3000 deaths. Although products such as fruits and vegetables are increasingly being implicated in foodborne illnesses related to Escherichia coli O157:H7 and Salmonella‚ red meat and poultry remain the predominant products contributing to outbreaks and recalls. Accurate and rapid detection of foodborne pathogens is a critical component of the process to reduce risks associated with pathogen-contaminated
Premium Escherichia coli Foodborne illness Bacteria
Running head: MULTI-STATE INFECTION Multi- State Escherichia coli O26 Infection Linked to Raw Clover Sprouts Escherichia coli are gram- negative bacteria that are normally found within the gut of warm bloodied organisms. There are several strains of E. coli that exist as part of the normal flora of the human digestive system. They prevent harmful bacteria from establishing themselves in the intestines‚ and they also aide in the production of vitamin K2. Most E. coli strains are not harmful
Premium Escherichia coli Bacteria
General information: What is E.coli? Escherichia coli‚ or commonly referred to as E.coli is a foodborne pathogen that normally inhabits in the intestinal track of humans and animals. It is the most common and wide spread infecting organism and is in the family‚ Enterobacteriaceae. Enterobacteriaceae is a large family of Gram-negative bacteria‚ meaning the bacteria appear pinkish or red. How fast and how does it spread? The main way that E.coli can spread is through food and water supply. Common
Premium Bacteria Immune system Microbiology
E. coli Escherichia coli‚ more commonly known as E. coli‚ are a large group of bacteria. It lives in the digestive tracts of humans and also animals. There are many different types‚ such as enterohemorrhagic E. coli (EHEC) which causes bloody diarrhea. Some types cause urinary tract infections. One other specific type that is real common is called E. coli O157:H7. This type can cause severe anemia‚ kidney failure‚ or even death. The way you contract E. coli is coming into contact with feces from
Premium Escherichia coli Bacteria