surrounding the colonies after addition of iodine on PDA plate. Organism Result + or - Appearance/Observations Bacillus cereus Escherichia coli B. Gelatin Hydrolysis (gelatinase production) Note: whether the gelatin deep remains solid or has been liquefied Organism Result + or - Appearance/Observations Bacillus subtilis Escherichia coli C. Coagualse: identification of Staphylococcus aureus Note: whether the rabbit plasma has remained liquid or become
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Aims The purpose of the two experiments was to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli‚ Pseudomonas fluorescens‚ Enterococcus faecalis‚ Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5‚ 25‚ 37‚ 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different
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jejuni colonies and microscopy * Campylobacter jejuni SEM * Capnocytophaga sp. * Chryseobacterium indologenes on Mueller-Hinton * Chryseobacterium indologenes colonies * Citrobacter freundii colonies on MacConkey * Escherichia coli on blood agar | * Escherichia coli on MacConkey *
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low price‚ and high degree of reduction have made glycerol a highly attractive and exploited carbon source for the production of fuels and reduced chemicals. Here we report the quantitative analysis of the fermentative metabolism of glycerol in Escherichia coli through the use of kinetic modeling and metabolic control analysis (MCA) to gain a better understanding of glycerol fermentation and identify key targets for genetic manipulation that could enhance product synthesis. The kinetics of glycerol
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Hydrolysis Report Bile Esculin Hydrolysis‚ Starch Hydrolysis‚ UreaHydrolysis‚ Casein Hydrolysis Hydrolysis Report Introduction Hydrolysis is a chemical reaction that uses water to split complex molecules. The water molecule H2O is split in the mechanism of hydrolysis‚ hydrogen cations and hydroxide anions. When the enzyme catalyzes its reaction inside the cell‚ it is referred to as intracellular hydrolases. When the enzymes secreted from the organism to catalyze reactions outside the cell‚ it
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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Unknown Lab Report #1 Unknown #1 April 25‚ 2012 Microbiology Spring 2012 MCB2010C Unknown #1 Introduction Identity of a microorganism has proven to be very significant. Doing so can help identify diseases and created treatment and cures for such diseases. As a result‚ various laboratory tests were performed to an unknown microbe (Unknown #1) found in the water of a nearby pond. By identify the microbe‚ the safety of the water will be known to those around it. Materials and Methods
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best indicator of faecal pollution (Edberg et al.‚ 2000). They also indicate the possible presence of pathogenic bacteria‚ viruses and protozoans (Kara et al.‚ 2004). Almost all the methods used to detect total coliforms‚ faecal coliforms and Escherichia coli are enumeration methods that are based on lactose fermentation (American Public Health Association‚ 1992). The Most Probable Number (MPN) method is a statistical‚ multi-step assay consisting of presumptive‚ confirmed and completed phases
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Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible
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sunlight or chemicals‚ nutritional requisites and biochemical actions. We have a patient that may be infected with an unknown bacterial infection; we’ve collected a sputum sample and will be testing for one of the following genera: Bacillus‚ Escherichia and Mycoplasma. In order to determine which one of the three may be the source of the infection we have to recognize the characteristics of each one and the staining protocol needed to identify them. All three of the above mentioned genera are
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