Lab Report: Antibiotic Evaluation by the Kirby-Bauer Method Introduction Chemical antimicrobial agents are chemical compounds capable of either inhibiting the growth of microorganisms or killing them outright. Those which are taken internally to alleviate the symptoms of or promote healing from disease are called chemotherapeutic drugs‚ and among these is a class of compounds called antibiotics. In order for a chemotherapeutic drug to be classed as an antibiotic‚ it must be produced by
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Introduction: Escherichia coli (E.coli) is a commensal-pathogenic organism which include a wide range of strains. There are several advanced molecular-genomic technologies that are capable for detecting and identifying different strains of E.coli. But‚ ERIC-PCR technique is a quick and cost effective method for determining individual strains via demonstration of strain specific fingerprint bands. Therefore‚ the ERIC-PCR technique was used to determine the isolated strains of E.coli from different
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Disposition/Food Safety: Overview of Food Microbiology July 8‚ 2011 Overview of Food Microbiology OBJECTIVES At the end of this module‚ you will be able to: 1. Explain the structural similarities and/or differences among Gram-positive and Gram-negative bacteria as well as their isolation and identification using serological‚ biochemical‚ and molecular techniques. 2. Identify the functions of the bacterial cell wall. 3. Identify the extrinsic and intrinsic parameters that affect bacterial growth
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Antimicrobial Activity of Cilantro on Staphylococcus Epidermis and Escherichia coli Abstract Antimicrobial effects of cilantro against the gram positive bacteria S. epidermis and the gram negative bacteria E. coli were looked at. We predicted that cilantro would act as an antibiotic against S. epidermis but not to E. coli because S. epidermis does not contain an outer membrane whereas E coli. does. We predicted that the semi-permeable outer membrane of E. coli would protect the bacteria from
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eventually slowing the aging process and diminishing ailments associated to aging (osteoarthritis‚ cardiac attacks‚ cancers‚ etc). Gastrointestinal Problems The use of Herbal extracts from Guava Leaves can slow down diarrhea due to bacteria such as Escherichia Coli‚ Salmonella Spp.‚ and Staphylococcus aureus. Guava Leaf extracts have also effectively treated diarrhea in patients with cholera. Weight loss Research has established the benefits of Guava Leaves for weight loss. Guava leaves work by prohibiting
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Title: Determination of a Bacteriophage Titer Purpose: To determine the number of phage particles or plaque-forming units in a suspension of T4 bacteriophage. Materials: 18 24 hour broth culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titer of at least 10‚000 phages/ml 5 trypticase soy agar (TSA) plates. These should be warmed to 37c before use 5 tubes of soft agar (0.7% agar). Prior to use‚ melt and hold at 50c in
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ENTERICS EMB‚ SSA‚ BSA [pic] Acronym: T LMI CUP MNL GARI ( TSI‚ LIA‚ MIO‚ Indole‚ Citrate‚ Urease‚ PAD‚ Malonate‚ Lactose‚ Glucose‚ Arabinose‚ Rhamnose‚ Inosite) easy steps to identify: *TSI: A/Ag------ Escherichia coli and Klebsiella pneumoniae A/Ag( yellow both slant and butt with gas: cracks/displacement of the agar) EMB- greenish metallic sheen ------ E. coli EMB- pink mucoid colonies -------- K. pneumoniae
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Materials 1) Microscope 2) Lab Coat 3) Gram Staining Lab 4) 2 Slides 5) Gram Stain Solution 6) Gram crystal violet 7) Gram iodine 8) Burner 9) Alcohol 10) Water 11) Staphylococcus epidermidis 12) Escherichia coli 13) Bacillus subtillis 14) Gram Safrain 15) Procedures First I get all my materials to get started. I make sure I turn on the burner and sterilize all my materials. I then smear E.C‚ BS‚ & SA and a mixture of all on 2 slides
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Main text. Questions. Page 3. 1. What does e.coli stand for? E.coli stands for Escherichia coli. 2. What are the symptoms associated with E.coli bacterial infection? The symptoms are diarrhea‚pain‚cramping‚nausea and vomiting. 3. give a detailed comparison of the levels of the e.coli bacteria over the five week period as given above. Page 4. 4. What could possible reasons
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protein in media was broken down to alkaline end product | Shigella is a genus of gamma proteobacteria in the family Enterobacteriaceae. Shigellae are Gram-negative‚ nonmotile‚ non-spore forming‚ rod-shaped bacteria‚ very closely related to Escherichia Shigella were discovered over 100 years ago by the Japanese microbiologist‚ Shiga‚ for whom the genus is named. There are four species of Shigella: S. boydii‚ S. dysenteriae‚ S. flexneri‚ and S. sonnei. Shigella sonnei‚ also known as Group D Shigella
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