2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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This lab is adapted from the canned denatured pineapple experiment. Instead of investigating the effect of canning on enzyme activity‚ we look for the presence of the substrate. This is a useful teaching lab for all the criteria and requires very few resources. Background Information: Gelatin is made from a protein called collagen which comes from the joints of animals. Gelatin may be dissolved in hot water. As the dissolved gelatin mixture cools‚ the collagen forms into a matrix that
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Part B: Practical Report The Effect of Temperature on Enzyme Activity Aim: To investigate how temperature effects the enzyme catalase. Hypothesis: If the temperature of water is increased then the enzyme will react quicker to form oxygen and water‚ when compared to cold water. Purpose: To design and conduct a plan of a practical about the effects of temperature on enzymatic activity with a partner. Introduction: An enzyme is a protein‚ which speeds up a specific chemical reaction without altering
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Jennifer Anatomy and Physiology Enzyme Lab Report Introduction The chemical reaction that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat
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Name __________________________________ AP Biology Period _________ Date ______________________ AP: LAB-RELATED AP EXAM ESSAYS LAB 1. OSMOSIS AND DIFFUSION ESSAY 1992 A laboratory assistant prepared solutions of 0.8 M‚ 0.6 M‚ 0.4 M‚ and 0.2 M sucrose‚ but forgot to label them. After realizing the error‚ the assistant randomly labeled the flasks containing these four unknown solutions as flask A‚ flask B‚ flask C‚ and flask D. Design an experiment‚ based on the principles of diffusion
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Biology “Enzyme Activities” Introduction: Enzymes have extremely interesting properties that make them little chemical-reaction machines. The purpose of an enzyme in a cell is to allow the cell to carry out chemical reactions very quickly. These reactions allow the cell to build things or take things apart as needed. This is how a cell grows and reproduces. At the most basic level‚ a cell is really a little bag full of chemical reactions that are made possible by enzymes (Brain). Laboratory
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Introduction Enzymes are protein based structures that help speed up chemical reactions. They help these reactions keep up with the everyday metabolic needs and other like functions of organisms. Enzymes are also considered catalysts‚ due to the lowering in activation energy‚ in which they are not consumed or changed at any point during the reaction. These enzymes have three main protein structures that help keep them formed and intact. Stage 1 of these structures is the primary structure‚ which
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two Title: Enzyme Function Purpose: To observe the role of enzymes in chemical reactions and to determine the kinds of cells that contain more of the enzyme catalase. Prior Knowledge: Enzymes are proteins that assist the chemical reactions of a cell by lowering the amount of activation energy needed to start the reactions‚ thereby enabling the cell to carry out the reactions at a faster rate; enzymes that lower the activation energy are therefore called catalysts. Moreover‚ the enzyme itself is
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Ye Tao BISC220-13155 The Effect of Temperature on the Digestion of Starch by Activity of Enzyme α-Amylase: Observation of Rate of Starch Disappearance through Iodine Test Introduction An enzyme is a type of protein that‚ through its own structure including hydrogen bonds‚ acts like a biological catalyst and is able to accelerate the biochemical reaction rate by lowering the activation energy of the whole process‚ without which cells could hardly practice any physiological functions within human
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