Investigating the effects of changing temperature on the activity of enzymes Background information: Renin is an enzyme that catalyses the coagulation of milk. It is found in the stomach of many animals and is used in making cheeses and junkets. It is found in the gastric juices or gastric mucosa of many mammals‚ including humans. In the human stomach‚ particularly those of infants‚ rennin works to curdle milk so that pepsin‚ another stomach enzyme‚ can further breakdown the proteins into absorbable amino
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ENZYME COFACTORS AND INHIBITORS 1. DESCRIBE THE GENERAL ROLE OF COFACTORS IN ENZYME ACTIVITY. Some chemicals enhance an enzyme’s activity‚ which is what cofactors function to do. They are a non-protein component of an enzyme and may be organic molecules (called coenzymes) or inorganic ions. 2. (A) NAME FOUR HEAVY METALS THAT ARE TOXIC TO HUMANS. Four heavy metals that are toxic to humans are: 1. Cadmium (Cd) 2. Lead (Pb) 3. Mercury (Hg) 4. Arsenic (As) (B) EXPLAIN IN GENERAL TERMS WHY THESE
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Lambda DNA with restriction enzymes. Aim: The objectives of this experiment are: Become more familiar with using micropipettes. Use restriction enzymes to cut DNA at specific sites. Use Ligase to rejoin some of the cut/separated DNA fragments. Learn to separate DNA using electrophoresis. Introduction: Restriction enzymes are proteins which cut dsDNA at specific regions depending on the enzyme used‚ determined by the nucleotide sequence of the DNA‚ i.e. each enzyme recognises specific nucleic
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Lecture 3: Enzyme kinetics Tue 17 Jan 2006 with the collaboration of Luna De Ferrari 1 Images from: D. L. Nelson‚ Lehninger Principles of Biochemistry‚ IV Edition‚ W. H. Freeman ed. A. Cornish-Bowden Fundamentals of Enzyme Kinetics‚ Portland Press‚ 2004 A. Cornish-Bowden Enzyme Kinetics‚ IRL Press‚ 1988 Computational Systems Biology Summary: • • • • • • 2 Simple enzyme kinetics Steady-state rate equations Reactions of two substrates Inhibition of enzyme activity pH
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The activity of an Enzyme is affected by its environmental conditions and changing these factors can alter the overall rate of the reaction. Reaction rates are influenced by external factors such as pH‚ temperature and salt concentration. Different enzymes have different optimum temperatures in which they are most efficient and different pH levels which is ideal for their formation of enzyme-substrate complexes. As the temperature increases‚ there is more movement of molecules and more random collisions
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reaction involves enzymes. So to understand how bleach works‚ we first understand how enzymes work. Enzymes Enzymes are the most important thing in our lives‚ it makes the world go ‘round! Enzymes are proteins that acts as catalysts and help combine or break down hydrogen peroxide. Imagine a pen with ink inside it‚ when you write‚ ink comes out‚ right? You can draw‚ you can write‚ then when you finish and get your pen off of the paper‚ the ink stops coming out. Enzymes are like that‚ they
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Melanie McGivern. Access to nursing Group 2 Effects of pH on enzyme activity Contents Front cover Aim Introduction Hypothesis Prediction Variables Materials Methods Results Discussion Conclusion Bibliography Aim The aim of the experiment is to see the enzyme amylase catalyse starch in a chemical reaction. | | Introduction Enzymes are proteins. They act as catalysts‚ allowing chemical reactions to take
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Matthew Saldanha Bio DCP lab-Catalase experiment Aim: To investigate enzyme kinetics‚ using different concentration of the enzyme. Hypothesis: The assay system used in the lab consists of a filter paper disc coated with the enzyme and the dropped into a papercup of substrate (Hydrogen Peroxide). As the hydrogen breaks down the hydrogen peroxide into hydrogen and oxygen gas‚ the bubbles of oxygen gas collect underneath the filter and make it
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| [pic] Introduction Enzymes are catalysts which speed up a reaction by providing an alternative reaction pathway of lower activation energy. But they do not undergo permanent changes and so they remain unchanged even at the end of the reaction. Many enzymes consist of proteins and non-proteins called the cofactor. The intra- and intermolecular bonds that hold proteins in their secondary
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process to anabolic hormones of the human body. Intrinsically they have like outcomes on the human body to anabolic steroids‚ triggering fast weight and force advances‚ but of a minor scale cause of the frequency limiting consequence produced by the enzyme adaptation. However‚ this theoretical explanation is thought-out somewhat outdated due to the expansions which have been done in supplement science. Epistane is amongst the hottest upscale steroids obtainable today‚ and it is achieving attention very
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