MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________ EXPERIMENT 1 TITLE: Observing Bacteria and Blood OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood. PROCEDURES: Using the microscope‚ an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood: After reviewing the section of the manual
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Potato Catalysis Lab Pre-Lab Assignment Background Information: An enzyme is catalytic protein. It is the most important type of molecule found in living cells. Cells would not be able to function without enzymes. Enzymes speed up or slow down chemical reactions of the cells. It is usually easy to identify the names of enzymes because they end in -ase. The enzyme that acts upon the substrate hydrogen peroxide is usually called catalase. This enzyme is found in both plants and animals
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Tittle : Investigation of the Enzymatic Effects of Materials on Hydrogen Peroxide Solution Objective: To investigates the enzymatic effect of various materials in the hydrogen peroxide solution. Table 1 Test Tube Contents with 5 cm3 hydrogen peroxide Observations before and after using wood splint Observation of after Observation of after adding hydrogen using wooden glowing peroxide splinter 1 Fresh liver Moderate
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point for the enzyme (catalase) to substrate (H2O2(aq)) concentration ratio. Thus‚ to truly understand this‚ the trial time period should be extended insofar that a declination in the rate of the reaction can be observed with multiple trials. If the trends of the independent trials coincide with one another‚ then it is plausible that a saturation point may have been a factor of the linear-like trend. This case will be further discussed in one of the five major factors that influence enzyme activity:
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being digested with EcoRI restriction endonucleasse. Procedures: λ DNA and puC18 DNA were put into two tubes respectively. Then‚ EcoRI buffer‚ EcoRI enzyme and deionized water would be put into both tubes. EcoRI enzyme was the restriction enzyme that cut the DNA at the specific sequence. The EcoRI buffer enhanced the stability of many enzymes and binds contaminants that may be present in DNA preparations. DI water was used to bring the solution into a required volume for gel electrophoresis.
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In the first part of the lab‚ we prepared a salivary amylase solution and aggregated acetic acid to detect the presence of mucin. Then‚ 4 test tubes were made of the following: Tube 1 = 3 ml starch + water + 37 degrees water bath; Tube 2 = 3 ml starch + saliva in water bath; Tube
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I also hypnotized that the enzyme is acidic on the pH scale because it exists in our small intestine and our small intestine uses acid to break down food. The result from the lab prove my hypothesis correct because lactase worked well at body temperature and it also worked well at pH of 4.5 to 5.5 mg/dl proving that lactase works best at an acidic pH number. The lab shows that the highest amount of glucose is made by temperatures close to body temperature. In our our lab‚ we found that at 35 °C 500
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ABSTRACT: This lab allows us to observe the conversion of hydrogen peroxide (H2O2) into water and oxygen gas. An enzyme known as catalase facilitates this decomposition reaction. The catalase enzyme acts as catalysis‚ helping lower the energy needed to activate the reaction while the enzyme itself is not affected. Catalase is a digestive enzyme used to break down hydrogen peroxide‚ which is a normal byproduct of cellular respiration. The reaction could take place without the help of catalase‚ but
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amylase lab‚ it was being tested if amylase‚ an enzyme found in saliva‚ would be denatured by being put in an acid or high temperatures. This lab is about denaturing amylase. It is tested by exposing it to pH and temperature changes. It is then mixed with Benedict’s solution‚ is a solution that changes color from blue to reddish brown when maltose is present. Amylase breaks starch into maltose‚ so is the amylase isn’t denatured‚ it should change colors. Amylase is an enzyme. Enzymes are a type
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system Tongue • For taste • Pushing food into the esophagus Papillae • Rough edges of that tongue that contain the taste buds Hard and soft palate • Separate the nasopharynx from the mouth Parotid gland • Secretes saliva (amylase enzyme) which breaks down starches into sugar Stomach • Stores food • Begins digestion of tissues and proteins by secretion of gastric juices. These juices are very acidic (pH 1-2). The semi-digested stomach contents are called chyme Esophagus
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