The Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of
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In order to get this‚ the stock solution‚ which was 3%‚ was diluted using 10 mL of hydrogen peroxide and 20 mL of water. In the second beaker‚ a ¼ dilution of potato extract was made using 2 mL of potato extract and 6 mL of water. The third plastic beaker contained 8 mL of water. Using the first and second beaker the experimental assay was performed. Using forceps a small paper disk was dipped into the second beaker containing the potato catalase for exactly 5 seconds. It was next dried on
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Lab Report on Osmosis and Diffusion Biology 1‚ Period 3 March 15‚ 2010 Lab Team: Jason Perez‚ Kicia Long‚ Chris McLemore Purpose: The purpose of this lab is to observe the acts of passive transport: diffusion and osmosis in a model membrane system. The experiment will show how molecules in solution move from areas of higher concentration to areas of lower concentration. The model membrane is dialysis tubing. Materials Used 2.5 cm dialysis tubing 15% glucose
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the effect of sugar on the mass of potato Introduction- diffusion is a passive transport where molecules move from areas of higher concentration to areas of low concentrations. Although the molecule ’s movement is random‚ equilibrium is being sought out. Osmosis is a type of diffusion only It involves water. In osmosis water diffuses from the area of high concentration to an area of low concentration‚ to diffuse the more molecule rich side. Because cells have selectively permeable membranes‚ meaning
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and air as the control. When performing this experiment‚ the main element to consider is hypertonic‚ hypotonic and isotonic solutions and how they relate to osmosis. Osmosis is the process where a fluid passes through a semipermeable membrane‚ which moves from an area which the solute is present in high concentrations. As a result of osmosis‚ there will be an equal amount of fluid on either side of the barrier‚ creating an isotonic solution. When cells are placed in an isotonic solution‚ the movement
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The purpose of this lab was to observe the rate of osmosis and diffusion‚ as well as the effect of molecular size of the particles on this rate. Part I of the lab was a demonstration of osmosis and diffusion‚ that dealt with raisins in different liquid environments‚ each with a different concentration of sugar. Part IV of the lab was using the same idea as the demonstration‚ by putting objects in different concentrations of a substance; in this case elodea leaves in salt water. In both cases‚ the
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Consequently‚ osmosis is leveling the concentrations on both sides of the membrane. However‚ the higher the temperature is‚ the higher osmosis will perform within the plant cell. When the molecules are heated‚ they start to move faster‚ causing osmosis to perform faster than at a neutral temperature. Temperature is just one of the many factors that can affect osmosis‚ such as‚ the pressure (the less pressure‚ the molecules will move leisurely
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the vial. 3. Drop one Clinitest® tablet into the vial. Place the vial on the tabletop and observe the reaction. Caution: the glass vial will become very hot during the chemical reaction. 4. After the reaction has stopped‚ wait 15 seconds. Shake gently to mix the contents. Caution: do not allow the contents of the vial to come into contact with your skin or eyes. Compare the color to the Clinitest® color chart. 5. Record the results of the test for glucose (positive or negative) in Table 1. Glucose
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Osmosis Lab Report by Evan Gerber Claire Cambron First Lab Report Wednesday 10:30am February 20‚ 2013 Theresa Gburek Abstract The major objective of the experiment was to test the effect of the concentration gradient on the diffusion rate. It was hypothesized that the greater the stronger the concentration gradient‚ the faster the rate of diffusion would be. To test this‚ dialysis tubes were submerged in different concentration fructose solutions. We weighed the tubes at specific
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Introduction In Diffusion‚ Dialysis & Osmosis lab‚ we discovered that we were going to observe and understand the conditions under which diffusion‚ dialysis‚ and osmosis occur. Then we constructed models of diffusion‚ dialysis‚ and osmosis. We predicted that the direction of change would be from the membrane to the beaker. We understood how these processes affected the selectivity of the cell membrane. The second part of the lab was dealing with dialysis. In this part we studied starch and sodium
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