Gel filtration chromatography on Sephadex G-50 The crude broth obtained after fermentation was subjected to ammonium sulphate precipitation at 70% (w/v). The pellet so obtained was resuspended in cold saline (2 ml) and dialysed. The dialysed enzyme was loaded onto a column of Sephadex G-50 (120 cm × 1.0 cm) equilibrated with 10 mM Tris-HCl buffer‚ pH 8. The column was eluted at a flow rate of 1 ml / 6 min. The elution profile of gel filtration chromatography is shown in the (Fig: 1). The fractions
Premium Molecular biology Protein Enzyme
Mobile phone-based money transfer Name Institution In recent years‚ there have been numerous milestones in technological advancements such as electronic word processing‚ social networking‚ instant messaging‚ Blue-ray‚ smartphones‚ search engines‚ Satellite TV‚ Wi-Fi‚ lithium rechargeable batteries‚ online stock trading‚ Bluetooth‚ DNA profiling‚ genetic sequencing and many others. These innovations help in our day to day lives especially in the fields of communication
Premium Mobile phone Bluetooth Internet
The word spondee is short for the term spondaic words. In audiology‚ spondees refer to a two syllable word such as cupcake and butterfly. In the textbook titled Introduction to Audiology‚ Martin defines the term spondee as a “two syllable word pronounced with equal stress on both syllables” (Martin 450). They are often seen in the audiological test battery by being presented to the patient either through a microphone or a voiced disk that has already been recorded and stored on an audiologist’s drive
Premium Phonology Phoneme
Six Word Memoir Narrative Football requires determination‚ perseverance‚ and heart. These three things are what separates average players from great players‚ mediocre teams from excellent teams. Those six words not only describe football‚ but my look on life as well. In life‚ we all need determination‚ perseverance‚ and heart to succeed in life as well as football. Determination is having the willpower to work hard to achieve a specific goal. When I am determined to achieve a goal‚ I work hard
Premium American football Game High school
Joshua English 1/Dr. Ivanova Intro Unit Essay Rough Draft The Horrible Word Dude Has anyone ever called you “dude?” How did it make you feel‚ and do you even know the meaning behind the word? What if‚ as time goes on‚ the use of the word dude becomes widespread; almost an epidemic on the World? Young children forget words like Mr and Mrs‚ Sir and Ma’am‚ and just call everyone dude. As time goes on these “children of the dude” grow up and take over the World. War breaks out because one
Premium Meaning of life Slang English language
50 Year Vietnam Memorial I was asked to talk about the 50-year anniversary of the Vietnam war. And as most of you can probably tell‚ I wasn’t alive 50 years ago‚ so I went to my grandfather who fought in that war and I asked him to recount his experience. I asked him if he lost any friends during that time. He responded with “Hunny‚ all the guys you are with are your friends. And it hurts to lose any of them.” He recalled for me one individual. Greg. He said “losing that one was hard.” He told me
Premium Family Vietnam War Vietnam
Lujain Alyahya Dr. Luhrs. PSY 105 Memory in Film I will be writing about 50 First Dates starring Adam Sandler and Drew Barrymore. This movie is about a girl who gets into an accident that results in brain damage. Then she experiences a form of amnesia called “Goldfield’s Syndrome” according to the movie. Basically‚ Goldfields syndrome doesn’t allow Drew to form new memories‚ but doesn’t erase what she knew before the accident. She can remember up to one day‚ then she relives the next day as if
Premium Death Suicide Mental disorder
Word and Sentence Structure (1LIN404) The language which I will be using to analyse is Turkish. English translation- “The neighbour’s daughter has saved the black cat that the dogs bit in her garden.” Turkish translation – “Koms’nun kizi bahcesindeki kopeklerin isirdigi siyah kediyi kurtardi.” Komsu – neighbour’s ‚ this is a possession Kizi = daughter Bahce – sindeki = in her garden Kopeklerin = dog’s. Kopek is singular when ler is added to the word it becomes plural the in at the end
Premium Present tense Subject English language
What is your favorite word‚ and why? My favorite word is callipygian. It is a Greek word‚ originally derived in the 1640’s or 50’s to describe a statute of Aphrodite. It is an adjective. It means having a well-shaped buttocks. It’s essentially a more sophisticated way of saying “DAMN‚ look at that ba-donk-a-donk!” After reading that‚ you may think I am a perverted animal. Please‚ let me explain. It started when I was a junior in high school. My friend came rushing down the halls laughing‚ his
Premium Laughter Meaning of life
My topic of vaccinations included statistics and data. Research of journals and accepted documentation allowed for establishment of my credibility. When written as an essay‚ it reached an academic audience. Yet‚ in a letter context‚ it reached the intended audience‚ an elected official who may be passionate about the topic. A direct letter referencing the life and death matter of children‚ appealed to the official’s emotions
Premium Rhetoric