The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
Premium DNA Gene Molecular biology
The purpose of this lab was to find out the reaction of iron nails and CuSO4‚ copper sulfate solution. Before starting the experiment‚ there were two possible outcomes of the reactions. CuSO4 + Fe → FeSO4 + Cu or 3CuSo4 + 2Fe → Fe2(SO4)3 + 3Cu. The ion chart showed that iron can only make two charges‚ +2 and +3. As the liquid evaporated‚ the weight changed because the liquid added to the nail’s weight. In a real world situation‚ scientists can use ratios to determine how much of a substance that
Premium Chemistry Chemical reaction Copper
experiment the objective was to perform a Diels-Alder reaction using cyclopentadiene and maleic anhydride to synthesize the product‚ cis-Norbornene-5‚6-endo-dicarboxylic anhydride. The Diels-Alder reaction is one of the most important reactions in organic chemistry and was first investigated by Otto Diels and Kurt Alder in Germany. It is a [4+2] concerted cycloaddition reaction which involves a diene and a dienophile. The Diel-Alder reaction are mainly used for creating new carbon-carbon bonds‚ mostly
Premium Carbon Oxygen Chemistry
Discussion The purpose of this lab is to display the influence of retronasal olfaction when it comes to flavor. Humans have two different types of olfaction. Olfaction is the detection of chemicals in the air. These chemicals which are meant to activate odor receptors‚ are called odorants. Humans have approximately 20 million olfactory receptors‚ which is only a fraction compared to bloodhounds who have 4 billion. The first type of olfaction is called orthonasal olfaction which is inhaling. Here
Premium Olfaction Taste Sense
Soda and Mentos Reaction: Any kind of Soda and Mentos interaction will expedite a geyser of both carbon dioxide and foam. Soda has a few properties‚ for instance‚ an sweetener or an artificial flavouring‚ but it’s main factor that contributes to the reaction is carbonated water. Carbonated water is basically water with Carbon Dioxide compressed within it‚ it causes the drink to be fizzy and bubbly. This contributes to the reaction because it the fizz and bubbles are gas‚ which tries to escape from
Premium Carbon dioxide Water Carbonated water
test tube D and E. The metal spreader was then sterilized again for any future uses. These plates for C‚ D‚ E‚ and the streaking plate were left upside down‚ taped and labeled with the section and lab ID number and then incubated for 2 days at 30 degrees Celsius and refrigerated until the following lab
Premium Laboratory glassware Agar plate Petri dish
TITLE OF EXPERIMENT: An investigation of the iodine/propanone reaction. DATA COLLECTION AND PROCESSING : Chemical Equation: CH3COCH3 (aq) + I2 (aq) ( CH3COCH2I + H+ (aq) + I- (aq) Rate of reaction: R = V T Concentration of reactant after dilution : Volume of reactant x concentration of reactant Total volume of mixture Uncertainty for rate of reaction: R =( V + t ) x R V t Eg
Premium Chemical reaction Chemistry Reaction rate
Single Displacement Reaction The objective of this experiment was to observe evidence of a reaction and to determine the chemical equation‚ if there is a reaction. In order for a reaction to occur‚ a metal strip must have evidence of black or grey deposits on the surface of the metal once dipped into the solution. The reactivity of an element is related to its tendency to lose or gain electrons. Three metal strips that were used are copper‚ lead and zinc as well as the solutions of silver nitrate
Premium Chemistry Hydrogen Ion
mistake was made. Results Reaction time to a second word or non-word is influenced by the previous word or non-word. The null hypothesis stated that all pairs would have the same mean reaction time‚ regardless of whether the first string was a word or non-word. The alternative hypothesis stated that the mean reaction time for word-non-word pairs would be slower than non-word-word pairs‚ which would be equal to the mean reaction time for non-word-non-word pairs.
Premium Education Educational psychology School
The purpose of this review paper is to discuss the effect of temperature on enzyme- catalyzed reactions. This is relevant because many diseases can be diagnosed and controlled by the processes of enzyme activity (Worthington 2015). If more information is not found about enzyme activity and how it is affected‚ many diseases may go undiagnosed and uncontrolled. Temperature is a type measurement that does not only consist of heat. It is the measurement of the mean kinetic energy of any group of particles
Premium Enzyme Chemical reaction Catalysis