atom or a fluorescent dye is used as label on probe. Reverse Transcription Polymerase Chain Reaction (RT)-PCR Employing PCR-based procedure in analyzing RNA would require cDNA to be synthesized using reverse transcription. When reverse transcription and polymerase chain reaction are combined together‚ it will permit detection of low abundance RNAs. RT-PCR is distinct from Real Time Polymerase Chain Reaction (qPCR). The latter measures the amplification of DNA in quantitative way and it uses fluorescent
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Chemical Reaction Lab Well #1 CuCl2 + Al (shot) - Bubbling - Turning reddish-maroon - 33oC Well #2 CuCl2 + Al (foil) - Bubbling‚ but less than well #1 - Turning black - 28oC Well #3 CuCl2 + Zn - Turned black then red - No bubbling - 29oC Well #4 CuCl2 + NH4OH - Cloudy - No bubbling - 26oC Well #5 CuCl2 + NaCO3 - Not mixing with CuCl2 - Heterogeneous - 25oC Well #6 CuCl2 + AgNO3 - Cloudy - Top layer is white -29oC 1. The more pronounced reaction was the aluminum
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ELECTROPHILIC ADDITION PURPOSE The purpose of this lab is to learn how to synthesize 2-methyl-2-butene into 2-bromo-2-methylbutane using addition of hydrogen bromide. THEORY Chemical Compound Molecular Formula Molecular Weight Boiling Point Melting Point Density Amylene CH3CH=C(CH3)2 70.13 g/mol 36 oC -134 oC 0.662 g/ml Hydrobromic Acid HBr 80.91 g/mol 126 oC -11 oC 1.490 g/ml 2-Bromo-2-MethylButane C5H11Br 151.05 g/mol 107 oC 1.18 g/ml Sodium bicarbonate NaHCO3 84.007 g/mol Decomposes
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Chemical Reactions Chemical Change • reorganization • original substances form new substances with different formulas • may or may not involve a change of state • symbols used to describe chemical reaction are known as a chemical equation • Chemical equations do not have equal signs (=) they have an arrow Chemical Equations • Must follow the Law of Conservation of Matter • atoms can neither be created or destroyed during a chemical reaction • What
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In this story by Connie Jan Maraan‚ the protagonist of the story is a ghost who died when the Tayuman LRT Station suddenly collapsed and the train came falling down on his face. According to him‚ he has seen Christ because of that incident. -Summary provided by: images.1aot.multiply.multiplycontent.com/…/PHIL.LIT%20-… (first entry that appears in Google upon searching Ghost by Connie Jan Maraan) In Connie Jan Maraan’s story entitled “Ghost”‚ she applied various symbols to express what happened
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STATE OF THE NATION ADDRESS BENIGNO “NOYNOY”AQUINO lll President Benigno s. Aquino lll has just delivered his fourth State of The Nation address (SONA) at the session hall of The House of the Representatives‚ Batasang Pambansa Complex On July 22‚ 2013 Being a candidate and become President is very hard because he need to face and solve all the problems .He need to help people who are needed ‚ he need to response to the few question of people. For him being a president of the country is not
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(AMD) problems are the most important environmental problems in sulphide mines especially mines which contain pyrite. Oxidation of sulphides in contact with air and water produces sulphuric acid which reduces the pH of water. The result of these reactions is increase in the solubility of heavy metals and consequently pollution of water resources. The Sarcheshmeh copper mine is one of the largest porphyry copper mines in the world which is located in south-eastern Iran. Chemical analysis of over than
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Biology 104 Reaction Times Objectives: 1. Formulate and test hypotheses regarding reaction times. Introduction: Reaction time is a measure of how quickly an organism can respond to a particular stimulus. Reaction time has been widely studied‚ as its practical implications may be of great consequence‚ e.g. a slower than normal reaction time while driving can have grave results. Many factors have been shown to affect reaction times‚ including age‚ gender‚ physical fitness‚ fatigue‚ distraction
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9/25/13 Endothermic Reaction Lab Report The Effect Ammonium Nitrate has on the Temperature of Water During this lab students are going to mix water and ammonium nitrate one gram at a time and observe the change in temperature that is occurring. The students are then going to record the data on a table and later will be able to make a graph of the results. A hypothesis for this lab could be if ammonium nitrate is added to water then the water temperature will decrease. . Hypothesis: If
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Aldol In this preparative lab‚ an aldol (trans-p-anisalacetophenone) was produced from the reaction between p-anisaldehyde and acetophenone with the presence sodium hydroxide. The reaction also showed the importance of an enolate and the role it played in the mechanism. Sodium hydroxide acts as a catalyst in this experiment and is chosen because of its basic conditions and pH. The acetophenone carries an alpha hydrogen that has a pKa between 18 and 20. This alpha hydrogen is acidic because of its
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