The acid-fast stain is performed on samples to demonstrate the characteristic of acid fastness in certain bacteria. Acid fastness is a characteristic that is shared by just a few organisms‚ so staining to determine if organisms possess this trait is useful in microbial identification schemes. The Ziehl-Neelsen method has endured as a reliable and effective way to demonstrate the acid-fast bacteria. Materials: 18-20 nutrient hour agar slant culture of Staphyloccus aureus 4 day old nutrient
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Title: Estimation of Ascorbic acid Aim: To determine the ascorbic acid content of canned apple and orange juice To determine the volume of canned juice necessary to provide 75mg of Vitamin C Introduction: For the estimation of ascorbic acid‚ the method used involved the titration of the ascorbic acid‚ in the presence of a redox indicator 2‚6-dichlorophenol-indophenol (DCPIP) which acts as both an oxidant so as to oxidise the ascorbic acid to dehydroascorbic acid‚ and it acts as an indicator
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Lab 5 Acid/Base Extractions Introduction The purpose of this experiment is to separate either the organic base (amine) or organic acid (carboxylic acid) from a mixture that contains inorganic impurities (salt) by performing a liquid-liquid extraction and then taking a melting point. Key Experimental Details and Observations Our starting material‚ Compound B‚ was a fine white powder and weighed 0.535g. The final product was a shiny white sheet that resembled acrylic paint and weighed 0.109g
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Titration for Acetic Acid in Vinegar Purpose: The Primary Purpose of this Experiment is to Determine the Percent Content of Acetic Acid in a Household Bottle of President’s Choice Vinegar‚ using Titration Techniques. In addition‚ a Secondary Purpose for this Experiment that Derives Directly from the Primary Purpose is to Gain Hands On Experience in Titration Techniques‚ as a Vital Tool in our Quest to Understanding the Chemical Aspect of the World around Us. Procedure Conducting a Titration
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Exercise 10 Acid/Base Balance NAME 1. Match each of the definitions in Column A with the appropriate description in Column B. Column A Column B F 1. pH E 2. acid D 3. base A 4. acidosis B 5. alkalosis C 6. carbon dioxide a. condition in which the human body’s pH levels fall below 7.35 b. condition in which the human body’s pH levels rise above 7.45 c. mixes with water in the blood to form carbonic acid d. substance
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Acid and Base Titrations: Preparing Standardized Solutions Introduction: This experiment focuses on titrations of acids and bases. A titration depends on addition of a known volume of solution and is a type of volumetric analysis. Many titrations involve either acid-base reactions or oxidation-reduction reactions. In this experiment we do one of each. We monitor the pH of the reaction with the use of a color indicator. We also learn about the standardization of bases (NaOH) and acids (HCl) which
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reaction schemes should include molecular weights and any other important physical data for the reagents and products. Ex. Cyclohexanol was oxidized to cyclohexanone using sodium dichromate in the presence of sulfuric acid. * Note: you can download a free version of a structure editor from ACD labs‚ the PC version is called chemsketch‚ the Mac version is called marvinsketch. You should learn how to draw structures using these programs. Lab reports should look professional and they should not have hand
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include universal indicator‚ methyl orange‚ phenolphthalein and many more. Acids and bases are diverse in this world. They are everywhere ranging from foods to trees to rocks and many more. Indicators can be used to determine whether these substances are acidic‚ basic or neutral. Manufactured indicators are one type and the second type is natural indicators. Believe it or not‚ the majority of fruits‚ vegetables and flowers contain pigments which may be used as acid-base indicators. For example
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Determination of Vitamin C (Ascorbic acid) concentration in some of Commercial Products‚ by Redox Titration Mouhannad AL.-Hachamii Sadiq J. Baqir Saadon A.Aowda Fatima A. Hussein‚ Dep.of chemistry‚ college of science‚ Babylon University Muhammed K.Alasedi Ministry of Health Hilla city‚ Babylon Abstract The goal of this search is to determine the concentration of vitamin C (Ascorbic acid ) in some of a Commercial Products‚ Vegetables and Fruit Juices by Redox Titration. A redox
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PRODUCTION OF CITRIC ACID Introduction: Citric acid is a White solid‚C3H4OH(COOH)3‚soluble in water and slightly soluble in organic solvents‚ which melts at 153 oC. Aqueous solutions of citric acid are slightly more acidic than solutions of acetic acid. Traces of citric acid are found in numerous plants and animals‚ because it is a nearly universal intermediate product of metabolism. Large amount of the acid are found in the juice of citrus fruits‚ from which it is precipitated by the
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