Construction of a Standard Curve for the Determination of Protein Concentration 09/13/12 PCB 3023-007 Results Figure1: Standard curve. This graph was constructed from the fixed amounts of Bovine Serum Albumin (BSA). Varying amounts of BSA at a concentration of .5µ/µl were mixed with the water and 1mL of coomassie dye. The equation y=0.0259x +0.0511 represents the best fit line for a data set taken from µg of protein equals two to ten µg. Optical density (OD) was surveyed using a Mach
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Experiment to investigate the effect of concentration of electrolyte on the current of voltaic cell Aim The aim is to identify the relationship between the concentration of H2SO4 solution and the current of Cu-Zn voltaic cell. Hypothesis My hypothesis is that the greater the concentration of sulfuric acid the bigger the current of the voltaic cell. In a voltaic cell‚ chemical energy is concerted to electrical though the oxidation and reduction happen on the electrodes. Current will not flow
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Auschwitz concentration camp. Furthermore‚ it was three different types of camps that were brought together: concentration camp‚ extermination‚ and labor camp (“Auschwitz was the largest camp”). All three camps played a major part in the Nazi’s “final solution” (Berenbaum). There were also subcamps part of Auschwitz. In just two years‚ 44 subcamps were built (1942 to 1944). Auschwitz also had different leaders. The first of the three leaders who controlled all of the Auschwitz concentration camps was
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- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Effect of Substrate Concentration on Catalase Aim: An experiment to find out how the concentration of hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information: Enzymes such as catalase are globular protein molecules with catalytic properties. A catalyst is a substrate which can alter the rate of reaction without itself undergoing any permanent change. As they are not changed
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The purpose of the experiment conducted was to observe if a higher concentration or lower concentration gradient would affect the diffusion rate of the iodine moving through the starch concentration. This was shown to be an accurate experiment as with a higher concentration gradient the diffusion that did occur happened a lot faster and diffused more efficiently compared to no iodine solution and just water. This is due to the molecular collisions speeding up the experiment in the time period given
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The Effect of Salt Concentration on Osmosis in Potato Cells We should first explain what Osmosis is. It’s a passage of water from a region of high water concentration through membrane to a region of low water. Semi membrane is a very thin layer of material‚ which allows some things to pass through them but prevent other things from passing through. The cells allow small molecules like Oxygen‚ water‚ Carbon Dioxide‚ Ammonia. But will not allow larger molecules like Sucrose‚ Starch‚ or protein‚ etc
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Before I came to the concentration camp‚ it was easy to be with your family but I have been in the concentration came for almost 3 months. In the times I been in the camp‚ I met my best friend‚ Graciela. Ever since I been in the concentration camp‚ life has been hard for everyone. In the mornings‚ you are awakened by the kapos in the 4 in the morning where you must raise and find your shoes to work. If someone stole your shoes‚ the person who took them often means they are send to death
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water molecules from a region with high concentration to a region with low concentration. This movement must take place across a partially permeable membrane such as a cell wall‚ which lets smaller molecules such as water through but does not allow bigger molecules to pass through. This process will take place until it reaches the equilibrium state‚ which means that the water molecules will be distributed until there are no regions with high or low concentrations. Plant cells have a protective layer—called
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Analytical Chemistry Experiment 2b: Determination of the ASA Content of Aspirin Due Date: September 16‚ 2013 Experiment#: 2b Title: Determination of the ASA Content of Aspirin Aim: To determine the Molar Concentration of NaOH and HCl acid used in their Standardization processes and to determine the acetylsalicylic acid (ASA) content in Aspirin. Materials/Apparatus: materials used are the same as that outlined in the laboratory procedure prepared by the laboratory instructor. Procedure:
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involves measuring the absorbance of several concentrations of the pure substance or the "standard" substance determine relationship between concentration and absorbance compared results from unknowns How to use Linear Regression for Generating a Standard Curve? © 2010 by M. Olaveson UTSC 2 BIO A01F-Fall 2010 - ASSIGNMENT # 2 - Preparing a Standard Curve using Excel 2007 Assignment # 2 Analysis of Data from Lab 2-Exercise 2 Table 2.6. Protein in Test Tubes prepared for
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