Direct competitive cocaine immunoassay was performed in a transparent high binding 96 well microtiter plate. Each well was coated with 200 µL of 1mg/L anti-mouse IgG in PBS (phosphate buffered saline‚ 10 mM sodium dihydrogen phosphate‚ 70 mM sodium hydrogen phosphate‚ 145 mM sodium chloride‚ pH 7.6). The microtiter plate was left over night for shaking at 750 rpm. Therefore‚ the plate was washed three times with automatic 96-channel plate washer by washing buffer (45 M potassium dihydrogen orthophosphate
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The objective of this lab was to prepare n-butyl bromide or n-bromobutane‚ which is derived from an alcohol and an acid. In this case‚ n-butyl alcohol and sulfuric acid were the reagents. There were two methods of distillation that was involved in this experiment. The first was by reflux distillation‚ which is used to speed up a chemical reaction without having the reactants/ products evaporate or explode. Data Table 1 indicates the amount of each reagents that was prepared for the reflux apparatus
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Observations: Before performing the experiment‚ the following remarks were recorded: The mass of the candle was 59.2 grams. The measurement of the visible wick is 0.5 cm. The length of the candle is 16‚5 cm. The candle was pink in color. The circumference of the candle was 7.5 cm. The candle’s width was 2 cm. The state of the candle was solid. The candle was already used and burned. The base’s circumference was 8 cm. The candle is buoyant in water‚ The candle’s volume is 66 ml. The density of the
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The Roaring Twenties were a time period of revolution‚ meaning an extreme or sudden change in the way people live‚ act‚ and/or work. This was shown socially‚ technologically‚ and economically. With the introduction of a new role for women‚ new technology‚ introduction of credit. Although the 1920’s had some impacts from a conservation revolution‚ the majority of the revolution was innovated. While the Roaring Twenties was more of a revolution of innovation (change or new)‚ the 1920’s also was a
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Introduction Polymers are large macromolecules composed of many small repeating subunits (monomers) connected via covalent bonds. Both natural and synthetic polymers are created via polymerization of these small molecules (Chemistry 144 Writing Assignment information sheet). The chosen polymer‚ polytetrafluoroethylene (PTFE) in the form of Teflon®‚ is a synthetic fluoropolymer of tetrafluoroethylene (Wikipedia‚ 2015)‚ and is one of the most well-known brand names for PTFE based formulas‚ owned by
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Today in the 21st century we view alcohol differently than the ancient Greeks did.The connection between the Dionysus myth and the way alcohol is portrayed today is the common link beings that alcohol is consumed at parties and other group events. In the Dionysus myth Dionysus is important because he is the greek god of wine he was widely worshiped because he was viewed as kind and understanding. Today in the 21st century alcohol is portrayed as this thing that is needed to have fun when you are
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The experiment did not contain any form of reaction mechanism since no chemical identity had been changed. The lab demonstrates the use of chemical molecular behavior to isolate a particular set of molecules. Caffeine had already existed in the leaf itself but needed to be separated from the other chemicals. Caffeine’s chemical structure is relatively similar to the nucleic acid purine in that they use nitrogen and is bicyclic but lacks an alkene‚ amine and an amide. Caffeine has a solubility of
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Referring to the Baldini episode in the novel perfume‚ show how the author uses imagery words and structural devices to show us the importance of Baldini to the progress of the protagonist. Baldini’s episode in very important in the novel perfume‚ due to several reason‚ one being the fact Baldini is like a life changing character for Grenouille just like how Madam Gaillard was. This is because Baldini introduces the protagonist to the art of perfumery and teaches him how to measure out ingredients
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Lava Lamp Science Investigatory Project Submitted by: Keanu Kent B. Gargar IV – St. Ezekiel 10/7/13 Introduction A lava lamp (or Astro lamp) is a decorative novelty item‚ invented by British accountant Edward Craven-Walker in 1963. The lamp contains blobs of colored wax inside a glass vessel filled with clear liquid; the wax rises and falls as its density changes due to heating from an incandescent light bulb underneath the vessel. The appearance of the wax is suggestive
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EXTRACTION OF TRIMYRISTIN FROM NUTMEG: Paul I. PURPOSE: The purpose of this experiment was to illustrate the extraction of a solid natural product from its natural source by partition with the aid of an organic solvent. Extraction is the process by which a compound or mixtures of‚ is transferred by separation from one phase into another. In this case‚ the major triglyceride contains a single fatty acid identified as myristic acid. It is a substituent
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