DATE PERFORMED: JANUARY 6‚ 2011 SPECTROPHOTOMETRIC DETERMINATION OF THE EQUILIBRIUM CONSTANT OF A REACTION ABSTRACT The objective of the experiment was to determine the equilibrium constant of the reaction forming ferric thiocyanate through the use of Spectrophotometry. For the calibration‚ five standard solutions were prepared‚ then their respective absorbance values that were obtained through the use of the spectrophotometer‚ were plotted versus the concentration of the analyte so that a calibration
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Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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Determination of Manganese in Steel Submitted by: ------------------- Date of Submission: 27.03.11 Objectives: In steel production aside the iron‚ which is the primary source‚ some amounts of manganese is used together with carbon. The objective of this experiment is determining the manganese amount in the steel compound by using UNICAM UV-VIS Spectrometer. Theory: Many molecules absorb ultraviolet or visible light. The absorbance of a solution increases
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Chem-102 Lab Dr. Sobhi Experiment #3: Spectrophotometric Determination of Tartrazine Purpose The objectives of this lab include- illustrating the use of the spectrophotometer in chemical analysis‚ and generating a standard‚ or calibration curve‚ then using that curve to determine the value of an unknown substance. The spectrophotometer is one of the most powerful tools used in chemistry to find the concentration of substances in solution. It compares the colors of a known and an unknown solution
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Figure 1: Schematic illustration of the principle of Biacore SPR to measure the interaction between analyte molecules in the flow with immobilized receptor binding sites on the surface. A) SPR uses an optical method to measure changes in the refractive index near the surface of a thin gold film on a glass slide. At a critical angle of incident light on the surface-solution interface an electromagnetic field or evanescent wave is generated. This is detected as absorption of light and a decrease in
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Crystallinity of SAPO-5 With Molar Composition The given mixture composition (Al: 1.3 P: 0.8Si: 1.2TEA: 100H2O ; Al: 1.3P : 0.05Si: 1.2TEA: 100H2O ; Al: 1.3P : 0.05Si: 0.2TEA: 100H2O) is used to study the overall effect of silicon in AFI framework.The SiO2 magnitude is varied mole ratio from 0 to 1.0 of the given SiO2 to Al2 .The crystals SEM images that is obtained by using various contents of silicon are representing the sample produced in the absence of silicon in reactant gel is similar to the
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Implementation of a Chirped Pulse Fourier Transform Microwave (CP-FTMW) Spectrometer Abstract: Under the condition in which electric and magnetic fields are absent‚ rotational constants is a three molecular constants that substantiate polyatomic molecule. In this laboratory experiment at Missouri University of Science and Technology‚ we are going to collect rotational spectra by using 2 separate types of microwave spectrometers -- the FTMW (Fourier Transform Microwave) spectrometer and CP-FTMW
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Introduction Objectives Validate the Beer-Lambert law for KMnO4. To determine the pKa for an acid-base indicator To estimate the equilibrium constant for the formation of complex ion Fe(NSC)²ꭞ The function of part two of the experiment is to find the value of the constant K‚ in the following equilibrium constant: K=[Fe (NSC) ²⁺]/ [Feᶟ⁺] [NCS⁻]‚ while not disrupting the equilibrium. Theory For part one. The majority of chemical compounds are known to absorb UV or visible light. Depending
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Based on the data from the riboflavin spectrum scan‚ the maximum absorbance wavelength for this compound is 446 nm. This was the point between 390 nm and 500 nm at which the absorbance value (0.72) was the highest. A blank tube that has the components of the solution being examined except for the compound of interest is then used in combination to provide an even more accurate reading. Then‚ by using Beer’s Law‚ the molar extinction coefficient for riboflavin was able to be calculated: 14‚400 L/(moles*cm)
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Materials 1 spectroscope 1 incandescent bulb 1 hydrogen gas discharge tube 1 mercury discharge tube 1 nitrogen gas discharge tube 1 fluorescent light 1 neon gas discharge tube Solar light source 1 discharge tube apparatus Procedure The procedure of using a spectroscope was practiced with a fluorescent light source. The slit was pointed towards the source‚ and the spectroscope was shifted until the spectrum was clearly visible. The appearance of the spectrum from the fluorescent light
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