Introduction The aims of the lab session was to familiarize with the principles‚ operation and application of a light spectrophotometer. This report discusses an experiment to study the relationship of absorbance and concentration‚ the interaction of electromagnetic radiant energy(ERE) and matter which is an important aspect of the Beer-Lambert ’s Law. This law states that the absorption of a compound is directly proportional to the concentration of that particular compound. This is because (a &
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Introduction: Hypothesis: My hypothesis for this experiment‚ the study of the effects of different wavelengths on leaf pigments‚ if chlorophyll a absorbs blue-violet light‚ chlorophyll b absorbs blue light‚ carotene absorbs blue-green light‚ and xanthophyll absorbs blue light‚ then wavelengths of greatest absorption will be 400‚ 450‚ 500‚ and 450 respectively. Independent Variable: Wavelengths Dependent Variable: optimum wavelengths of light for absorption Standard Variable: method of
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Experiment # 9: Optical Method of Analysis Use of Beer’s Law on a KMn04 Gregorio‚ Justin Edrik A. March 2013 Department of Chemical Engineering‚ Faculty of Engineering University of Santo Tomas España‚ Manila Abstract The purpose of this analytical laboratory experiment is to determine the unknown concentration of potassium permanganate (KMnO4) solution by finding its absorbance through the use of spectrophotometer. The preparation of four known concentration of KMnO4 was done namely‚ 2
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ESTIMATION OF IRON IN IRON ORE-SPECTROPHOTOMETRIC METHOD By: Taylor Villari Experiment conducted on 7/22/13 Components of each test tube examined in the spectrophotometer Trial | Volume of Iron solution (mL) | Micrograms of Iron | Volume of 10% sodium acetate | Volume of 0.1% o-phenanthroline | Volume of water (mL) | 1(blank) | 0.0 | 0.0 | 1.0 mL | 1.0 mL | 8.0 mL | 2 | 1.0 mL | 10 | 1.0 mL | 1.0 mL | 7.0 mL | 3 | 3.0 mL | 30 | 1.0 mL | 1.0 mL | 5.0 mL | 4 | 5.0 mL | 50 | 1.0 mL
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Tittle : Absorption Spectra and the Beer-Lambert Law. ɛ Purpose : Understanding of Beer –Lambert Law Introduction : When light passes through the colour solution ‚ the molecules of the solution absorb the quantity of light at a particular wavelength . The amount of light which is absorbed by solution depend on two things. 1. The length of the pathway of light 2.Concentration of colour solution. These are all explained by the Beer-Lambert Law
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Flame Test Laboratory Report Introduction The purpose of this lab is to observe and identify metallic ions‚ using flame tests. The traditional flame test originated when Robert Bunsen invented the Bunsen burner to test two new alkali metals he and his partner had discovered. “A flame test is an analytic procedure used in chemistry to detect the presence of certain elements‚ primarily metal ions‚ based on each element’s characteristic emission spectrum” (Flame Test 1). Using this test‚ an element
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The spectrophotometer is an essential tool for biologists and chemists in analyzing chemical and biological samples. Gaining familiarity with its operating protocols and understanding what its outputs mean are very important in the development of lab technique for students of cell biology. This experiment will help laboratory students gain experience in using the spectrophotometer. Basic Laws of Light Absorption - For a uniform absorbing medium (solution: solvent and solute molecules that absorb
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Extraction and Quantitative Determination of B-Carotene Introduction The study of B-Carotene is important because it is good for vision‚ immunity‚ and overall health. The purpose of this experiment is to extract and find quantitative amount of Beta-carotene from a carrot sample. The raw product(carrots) was extracted using simple distillation. The purity of the product will be characterized using percent yield‚ thin layer chromatography‚ Beer’s Law‚ and UV-vis. Beer’s law is used because in this
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In experiment 3.1.We set up and calibrate the spectronic 20 first since it takes time to be ready to use so‚ we turned the spectronic 20 to warm up for at least 15 minutes‚ set wavelength to 360 while no test tube in it. Also‚ we set the filter lever in order to match the wavelength range‚ and then set the transmittance to 0%‚ then we changed the mode to absorbance. After we labeled two test tubes which were #5 and #10‚ we inserted water first to set 0 absorbance‚ then we used #5 sample to read it
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The data we collected and the calculations we performed demonstrated that the color of a given food dye depended on which light was transmitted in the visible spectrum and which light was absorbed. A quantitative measurement of the absorbance spectrum of each food dye was obtained through spectrophotometry.1 Through this method‚ we determined that the wavelength of maximum absorbance of a given food dye was directly correlated to the color of that food dye. Further‚ darker food dyes absorbed more
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