Methods We began this experiment by turning the Spectrophotometer to 605 nm absorbance‚ and setting up each of the tubes as stated in Table 7-2*‚ ignoring the DPIP until the rest of the sample was set up. Once all of the samples were set up accordingly‚ we blanked the spectrophotometer‚ and added the DPIP to the sample right before‚ inverting the sample and taking a reading at the Zero time mark‚ and placing the sample in the designated light apparatus‚ Sample 1 was wrapped in aluminum foil to
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1. The standard curve is a graph that shows a relationship between concentration and absorbance of a solution. A standard curve was experimentally created in this experiment using 10mL solutions of phenol red with concentrations 10µM‚ 7.5 µM‚ 5.0 µM and 2.5 µM then the absorbance of each sample was measured using a spectrophotometer. This generated curve with resulting average absorbances of 1.273nm‚ 1.0275nm‚ 0.585nm‚ 0.285nm and 0.124nm provided a means to determine the phenol red concentrations
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Observing Flames and Specs through a Spectroscope By: Seif Alkhouri Cristian Plancia-Mata Charlotte Maxwell Suryansh Kumar Evan Hatton J. Immel Ph.D Introduction Spectroscopy is the study of the interaction between matter and radiated energy. English philosopher Roger Bacon(1214-1294) was the first person to recognize that if sunlight passes through water it splits into colors. Around four centuries ago‚ Isaac Newton originated the title “spectrum” to label the shade of rainbows put
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M. S. Ntamila and A. Hassanali University of Dares Salaam P.O. BOX 35061 Tanzania kolation of Oil of Clove and Separation of Eugenol and Acetyl Eugenol . An instructive experiment for beginning chemistry undergraduates chloride‚ and hydroxamic ester tests‚ and the preparation of the henzovl derivative of eupenol. Finally‚ ir spectra of the two components are examined‚ and the information ohtained comoared with that from chemical tests. Practical Detalls Crush about 30-35 g of cloves with pestle
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Introduction: This investigation used spectroscopy to evaluate light absorption in different solutions. A spectrophotometer was used in the lab to determine these values. A spectrophotometer is an apparatus used to “measure the absorption of radiation in the visible and UV regions of the spectrum and allows precise at a particular wave length” (Jones et al.‚ 2007). The amount of light absorbed by a substance is directly in relation to the concentration of the solute and also the wavelength moving
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Abstract This report investigates the absorbance of methylene blue and carmine red using a spectrophotometer to determine the absorption spectra of both solutions. The concentration of the unknown solution of methylene blue was found to be 1.07 x 10-5 M by using the molar extinction coefficient‚ with absorption of 0.547. It was also found that the results concluded confirmed beer’s law with an R2 value of 0.9989. Introduction Spectrophotometry is the quantitative measurement of the absorbance
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This lab report will be detailing the steps taken and the results discovered when using spectrophotometry to determine the percentage of copper in a copper-clad penny and the thicknes of the copper layer on the copper-clad penny. After 1982‚ copper coating has been used in the creation of the penny because the cost of pure copper has increase to the point that the amount needed t omake a penny cost far more than the actual value of the penny. This lab allowed us to see just how much copper coating
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Introduction: Experiments 1 and 2 main aims are as follows: • To able to use the Beer-Lambert law to explain the relationship linking concentration and absorbance of the coloured solutions methyl orange and bromophenol blue. • To be able to rightly operate a spectrophotometer to assess the absorbance at various wavelengths • To be able to identify the main component parts of a spectrophotometer • To be able to draw a ‘Standard Curve’ from the data collected
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XRD – X-Ray Diffraction X-ray diffraction (XRD) is a powerful non-destructive technique for characterizing crystalline materials. It provides information on structures‚ phases‚ preferred crystal orientations (texture)‚ and other structural parameters‚ such as average grain size‚ crystallinity‚ strain‚ and crystal defects. X-ray diffraction peaks are produced by constructive interference of a monochromatic beam of X-rays scattered at specific angles from each set of lattice planes in a sample. The
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In the year 1803‚ Thomas Young first introduced the idea of trichromatic color representation. Young proposed that we have three “particles” in the eye that are each sensitive to a specific range of visible light that allows us to see color. Years later‚ Hermann von Helmholtz continued Young’s theory. Helmholtz suggested that the three different “particles” were categorized by their response the different wavelengths of light that hit the retina. Today‚ we know that the “particles” that Young and
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