Determining the Concentration of an Unknown Protein Solution with the use of the Bradford Method Paul Alcala‚ Andrea Basa‚ Melissa Caagbay‚ Frances Cayomba Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state
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P D Y Chapter 3 1 0 2 SURFACE MINING METHODS REFERENCE: BULLIVANT‚ DA. Current Surface Mining Techniques. Journal for the Transportation of Materials in Bulk: Bulk Solids Handling‚ vol 7‚ n6‚ December 1987‚ pp827-833. 2.1 Ore reserves Suitable for Surface Mining Ore reserves suitable for surface mining can be classified initially as; Relatively horizontal stratified reserves with a thin or thick covering of overburden Stratified vein-type deposits with an inclination steeper
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Define human error‚ and explain the HFACS method used to classify human error and how HFACS can be both reactive and proactive. Human error can simply be described as an error made by a human. But it gets a little more complicated than that. People make mistakes. However‚ understanding why they make mistakes is vital. With that in mind‚ let’s go ahead and explore this very question. As stated earlier‚ human error is a mistake that occurs due to a person’s failing to properly execute something
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The Simplex Method: Learning Team A Mike Smith‚ Todd Jones Math212/Introduction to Finite Mathematics February 1‚ 2011 The Simplex Method: Learning Team A Sam’s Hairbows and Accessories is a small company preparing for the next scheduled craft fair. The owners‚ Sam and Todd‚ both have full-time jobs in addition to owning the company so they are only able to spend a combined total of 80 hours labor to prepare for the fair in four weeks. Sam’s offers five main product lines: basic bows‚ elaborate
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Pilates Method for Lung Function and Functional Capacity in Obese Adults Joseph E. Mendoza Grand Canyon University BIO 201 Lab November 20‚ 2016 Pilates Method for Lung Function and Functional Capacity in Obese Adults Introduction Obesity is an extremely commonly condition in modern society. Obesity is defined as having a body mass index that is above 30 kg/m2. Obesity can affect the quality of life of an individual. It is estimated that about 2.8 million people die every year from causes
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Scientific Method The Scientific Method is a system devised to allow scientists to gain insight‚ or knowledge‚ on a wide range of questions about one’s behavior and other points of interest. The first step in this process is to identify a question that deserves an explanation or answer to. For example‚ a good indentifying question that one could use would be why did the chicken cross the road? This is a specific behavior that can be addressed and quantified. Once the individual has the question
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.(i=1‚2‚3‚4: j=A‚B‚C) Cij= Shipping cost per container from distribution center I to customer j Subject to‚ ∑XiA=600 ∑XiB=500 ∑XiC=500 ∑X1j=450 ∑X2j=600 ∑X3j=350 Xij= integer The VAM penalty cost is given by‚ The initial allocation in VAM method is made in the row or column that has the highest penalty cost. In following table‚ column C has highest penalty cost i.e. $350. and we choose cell 4C. Iteration 1) A B C Hongkong Singapore Taipei Supply Penalty 1 LA 300 210 340 450 90
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Question 2 Part (a) Table 1; Data Summary ∑f=80 Calculations The sample arithmetic mean x ̅=( ∑▒〖(MI)×f〗)/(∑▒f) =1535/80=19.19 The median x ̅ =〖 L〗_(1 )+( (N/2-∑▒〖fmed )×c〗)/fmed =15+((40-27))/((22))×5 = 15 + 2.95 = 17.95 〖 L〗_(1 )=Lower class limit of the median N=Total Frequency ∑▒█(fmed )=Sum of the frequencies of classes below the median class@) fmed =Frequency of the median class c=Width of the median class The mode (X ) ̂=〖 L〗_(1 )+ (f_(0-f_1 )/(2 f_0-f_(1
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METHODS OF IRRIGATION The manner in which water is applied to the land is commonly referred to as method of irrigation. These methods are adopted to apply irrigation water to the crop depending on the landscape‚ amount of water and equipment available‚ the crop and method of cultivation of crop. The main aim of these methods is to store water in the effective root zone uniformly and in maximum quantity ensuring minimum water loss and to get optimum yield. Various methods of irrigation are: 1.
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Describe one method you use to keep (a) staff and (b) students informed of what is happening in your organization. Staff The organization where I work consists of seventeen (17) staff members‚ ranging between the ages of twenty and sixty. Our school building is diverse‚ in that there is a two storey consisting of ten classrooms and a Literacy Room‚ as well as‚ two separate buildings at another end with four classrooms. Hence the reason why face-to- face communication is vital. As persons today
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