one outlier that was strange was the one paper towels. Only 14 of the 15 seeds germinated in that group. The difference that we could observation was the healthiness of the plants. IV. Conclusion Paragraph 1 Recap (Evidence): The purpose of our lab was to see if the amount paper towels would affect the germination of the seeds. My hypothesis was‚ if I increase the amount of paper towels‚ then the germination rate would increase too. We observed the petri dishes in class and we would add 2 ml of
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The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Introduction In this lab‚ the purpose was to verify Hess’s Law. Four main topics were covered during this experiment including enthalpy of reaction‚ heat of formation‚ Hess’s Law‚ and calorimetry. The enthalpy of reaction‚ ΔHrxn is the heat or enthalpy change for a chemical reaction. The energy change is equal to the amount of heat transferred at a constant pressure in the reaction. The change represents the difference in enthalpy of the products and the reactants and is independent of the steps
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are many conditions of the environment that can affect the optimum operation of enzymes. These condition include temperature‚ enzyme concentration‚ substrate concentration‚ acidity‚ salinity‚ and any present activators/inhibitors. In this particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes;
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According to the data from both the lab group and the class average‚ there is evidence that osmosis did occur in the bags. The largest change in mass was in the 1.0M sucrose bag the mass went from 12g initially to 14.2g‚ this gained 2.2g‚ an 18.3% change in mass for the group data over the duration of the experiment. The 0.2M bag went from 10.2g to 10.9g a 6.9% change in mass; the 0.4M bag went from 12.1g to 12.2g .83% change in mass; the 0.8M bag went from 10.9g to 12.2g and an 11.9% change in
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can survive in extreme environments. Halobacteria are also useful by being a good organism to perform DNA transcription‚ translation‚ and transformation on (Kramer‚ 2006). There are two different types of Halobacteria that are being observed in this lab. The first is NRC-1‚ which is also called the wild type strain. Although the pigmentation of the Halobacteria is caused by the production of the membrane protein‚ bacteriorhodopsin‚ which is a red‚ the wild type strain is pink in color. This pink color
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In the unknown identification labs‚ we have identified our unknown as Pseudomonas aeruginosa. Pseudomonas aeruginosa is Gram negative and rod shaped that we found to be motile in the lab. Our strain of P. aeruginosa formed colonies that were round in shape and had scalloped margins on nutrient agar. On our agar slant‚ the P. aeruginosa colonies had a filiform appearance on the edges. I think we correctly identified our unknown as P. aeruginosa because we performed several different tests‚ eleven
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In this lab‚ we extracted spinach pigments‚ and analyzed what colors of light these pigments absorb. By using TLC plate‚ hexane and acetone‚ I separated the pigments of spinach‚ and discovered that the main pigments were green and yellow. This works because with different polarities‚ pigments move at different rates. Hexane and acetone were also used to separate chlorophyll and carotene from spinach. Since they are polar‚ they can separate organic and inorganic things. From the experiment‚ I know
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Abby Goldschmidt Honors Biology 2° Mrs. Gempel September 3‚ 2015 Daphnia Lab Results Paper Abstract The goal of the study was to observe the effects of multiple chemicals on a Daphnia magna’s heart-rate compared to a control (pond water). The different chemicals were caffeine and alcohol. The heart-rate was the main variable in this experiment. The Daphnia’s heart-rate was observed for 15 seconds and then multiplied by 4 to show its heart-rate in one minute. This was repeated 4 times for each
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