Investigating the effect of changing substrate concentration on the activity of the enzyme catalase The aim of this experiment is to examine how the concentration of a substrate (hydrogen peroxide) affects the rate of reaction of an enzyme catalyse (found in liver cells) Research Question: how does changing the concentration of the substrate affect the rate of reaction of the enzyme catalyse? Hypothesis: As the concentration of the substrate increases‚ so does the rate of reaction until the reaction
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Enzyme Lab Introduction/ Abstract An enzyme is a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. They are mainly made up of proteins and can tremendously speed up reactions. E. coli ( a bacterium) has about 1‚000 different types of enzymes floating around in its cytoplasm at any given time. Enzymes can be used to join and even break up molecules as shown in the diagram below. (1)
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Restriction Enzymes Restriction enzymes (also known as restriction endonucleases) are a group of bacterial enzymes which cut double-stranded DNA (dsDNA) into smaller fragments at specific points. They are a defence mechanism used by bacteria to cleave the DNA of invading viruses‚ thereby restricting their expression. The exploitation of restriction enzymes ability to cut large pieces of DNA into smaller fragments (called restriction fragments) and the highly specific way in which they do this
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English 101 Mr. B 17 March 2013 Do you exercise? Obviously exercise is important and has many benefits. When the word exercise is mentioned‚ what comes to mind? Some people believe that exercise is more of a health and heart thing. Some believe exercise is more for appearance and the way one looks. While others believe exercise is for psychological reasons. Exercising is important for a lot of things dealing with the body. Most all benefits of exercise are helpful for the body and health.
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Effect of Temperature on Enzymes ~Abstract~ In this experiment the effect of different types of temperatures on enzyme activity was examined. The temperature baths that were used to test the difference in enzyme activity on fresh liver were; 4 °C‚ room temperature which was 22°C‚ body temperature which is 37°C‚ and 77°C. The total time of each trial was 2 and a half minute‚ 1 minute for the H2O2 to acclimatize to the temperature‚ 1 and a half minutes for the reaction to occur. Catalase causes Hydrogen
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Enzymes are biological catalysts that operate at the temperatures‚ pressures‚ and moderate pH values found in living organisms. They help in a faster chemical reaction in our human body. Besides this‚ enzymes also play a huge role in various industries. The following paragraphs will talk about how various enzymes are used in various industries : The Dairy Industry and Medical Industry. In the dairy industry‚ the enzyme Protease is used widely. While making cheese‚ protease helps coagulate milk
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Enzymes- reduce the amount of energy required for the reactions they catalyse [accelerate]. -thus‚ increasing the rate of reactions that occur in living organisms. WITHOUT enzymes : metabolism would be so slow at body temp. that insufficient energy would be available to sustain life. -Many enzymes are “Intracellular”- used within the cell that produced them e.g. enzymes in respiration & photosynthesis -Others are “extracellular”-they act outside the
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Questions 1. What did you observe before putting it in the refrigerator? Before putting the Jell-O in the refrigerator there was no change to the first two cups of Jell-O‚ however the Jell-O with the most tenderizer went slightly cloudy. 2. What did you observe after 1 hour in the refrigerator? After 1 hour of the Jell-O being refrigerated cup â»1 with no tenderizer was set‚ cup â»2 with the least amount of tenderizer was firm but not set‚ and cup â»3 with the most tenderizer was not set
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Title: Catalase enzyme detection Objective: To understand the function of catalase in cells that produce the enzyme‚ interpret the results of a catalase test and know their value in differentiating bacteria. Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of
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BIOLOGY HSC NOTES MAINTAINING A BALANCE • Identify the role of enzymes in metabolism‚ describe their chemical composition and use a simple model to describe their specificity on substrates. Enzymes are protein molecules that allow the body to engage in chemical reactions‚ such as metabolism. There activities can be catalytic (being able to control the rate of either increasing/decreasing chemical reaction) Enzymes have a specific shape‚ and this shape must be intact‚ otherwise the effectiveness
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