15th Influence of pH on Enzyme Activities How does the pH of solution influence the speed of enzyme activities? Enzymes are protein catalysts and chemical that speed up chemical reaction without being involved in the reaction. They are involved in the human digestive system to help people digest food more efficient. Food are substrates to be break down and absorb through the digestive system in the body. Substrate is a molecule that bond with the active site of enzyme to transfer in to one or
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Group No. Name: | Date Performed: | Course & Section: | Date Submitted: | Program & Year: | Professor: | Experiment 1 GASLAWS A. Combined Gas Law Temperature of Boiling Water in Kelvin (T1) | | Temperature of Cold Water in Kelvin (T2) | | Atmospheric Pressure in mm Hg (P1) | | Vapor Pressure of Water at T2 in mm Hg | | Final Pressure of Air in mm Hg (P2) | | Volume of Water Collected in the Flask in mL (Vwater) | | Initial Volume of Dry Air in mL (V1) |
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Enzymes Abstract: The following 2 labs experimented the more enzymes and substrates added to the concentration will effect the reaction rate. Our second lab‚ we tested enzyme and substrate concentrations to determine the increase of temperature and inhibitor. The enzyme source used in both labs was peroxide‚ guaiacol is used as a substrate for peroxide. We used Guaiacol‚ turnip extract‚ peroxide and distilled water for enzyme and substrate concentration. In the second lab we used the same substances
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Lab Report Effect of temperature on enzyme activity I. Purpose: Determine how effect of temperature on enzyme (catalyse) activity (in the liver). II. Materials * Raw liver * Forceps * 50mL 1% hydrogen Peroxide (H * 25mL graduated cylinder * 50mL‚ 400mL beakers * Pureed liver * 5 Filter-paper disks * Paper towels * Timer or stopwatch * Thermometer * Hot plate III. Procedure 1.Gather all necessary materials; start
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Experiment 2: Estimation of protein concentration Name: Wong Jiun Hao ID: 00000011136 Cohort: BM114 Module: Biological Science Table of Contents Title 1 Table of Contents 2 Introduction 3 Objectives 4 Procedure 4 Results 5-6 Discussion 7-8 Conclusion 9 References 9 Introduction The concentration of protein in a sample can be obtained by using the Bradford protein assay method. The method requires a spectrophotometer
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Determining the Concentration of a Solution: Beer’s Law Purpose: The purpose of the experiment is to determine the concentration and formula of an unknown cobalt nitrate solution by measuring absorbance. Introduction: A Colorimeter will be used to determine the concentration and formula of an unknown cobalt nitrate solution. The colorimeter sends blue light from the LED light source to pass through the solution and hit a photocell. A solution with a higher concentration will absorb more
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Principles of Biology Lab Exercise Enzymes: Catalysts of Life Instructor: Professor Alcendor By Shahid Rana Date: March 7th‚ 2013 Abstract: In this experiment we have demonstrated the function of enzymes. The whole experiment was devoted to understand how enzymes work as a catalysts and increase the chemical reaction without being used themselves. In general‚ enzymes are proteins that function as biological catalysts. These enzymes adhere to lower to amount of energy required for
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the
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Purpose: Restriction enzymes cut DNA at a certain palindromic sequence. Three samples of lamda DNA set up to be cut with restriction enzymes PstI‚ EcoRI‚ or HindDIII. There were also two more samples‚ one of these samples was not mixed with any restriction enzyme and the other was a marker‚ which used an enzyme which creates fragments with a known number of base pairs used to create a standard curve. All five samples were put through agarose gel electrophoresis in order to estimate the amount of
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Strengths of Materials Lab #4 CIVENG 303 Section 804 Stress Concentrations Performed October 24th‚ 2013 Today’s Date: November 7th‚ 2013 Tim Hendrickson OBJECTIVE The objective of this lab is to determine a set of changes due to the stress in geometric irregularities in an axially loaded bar. Some of the irregularities include certain features such as holes and notches. QUESTIONS: -The following are formulas are for stress and strain that are biaxial. Sample Material
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