Methyl Red and Voges-Proskauer Test The Methyl Red test shows which bacteria are creating stable acids through mixed acid fermentation of glucose. This helps to identify enteric bacteria by examining how they metabolise glucose. Every enteric bacteria first produces pyruvic acid from metabolism of glucose. A methyl red positive enteric bacteria‚ uses the mixed acid pathway when breaking down pyruvic acid to different acids‚ like lactic‚ acetic‚ and formic acids. The Voges-Proskauer test determines
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In this experiment‚ we used methyl benzoate from the last experiment with HNO3 and H2SO4 to synthesize methyl 3-nitrobenzoate. First we added methyl benzoate to 12 mL cooled conc. Sulfuric acid in a flask. In a separate flask‚ we made a solution of 4 mL conc. Sulfuric acid and 4 mL nitric acid and then added dropwise to the solution of methyl benzoate in an ice bath on a stir plate while maintaining the temperature of reaction between 5-15 °C. After the addition was complete we took the flask out
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Experiment 4A: Determination of a Partition Coefficient for Benzoic Acid in Methylene Chloride and Water Experiment 4B: Solvent Extraction I: Acid-Base Extraction Using the System Benzoic Acid‚ Methylene Chloride‚ and Sodium Bicarbonate Solution Objective A: To accustom participants (students) to general procedures that are used to obtain a partition coefficient at the microscale level. We will gain experience in such practices as the transfer of microliter volumes of solutions with a Pasteur filter
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EXPERIMENT NO. 6 DETERMINATION OF THE MOLAR VOLUME OF A GAS AND THE UNIVERSAL GAS CONSTANT Salve‚ Ryan Angelo TAB3‚ Group 6‚ Mr. John Kevin Paulo Biadomang Tabor‚ Frances Hermilyn March 8‚ 2013 ------------------------------------------------- I. Abstract This experiment is working with the ideal gas law‚ which is the summation of Boyle’s Law‚ where pressure is inversely proportional to volume‚ Charles’ Law‚ where the volume is directly proportional to temperature and Avogadro’s Law‚ where
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Full Lab Report Experiment #2: Acid-Base Titration Lab Description: Acid-Base Titration Introduction In this lab exercise we will evaluate the effectiveness of several indicators for the determination of the point of completion of a specific acid-base neutralization reaction. We will also determine the unknown concentration of the strong base NaOH by its reaction with a known amount of the weak acid‚ potassium acid phtalate (HKC8H4O4‚ abbreviated KHP). This will be accomplished using the titration
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General Chemistry II Lab (CHEM 1106) Determination of the Solubility Product Constant of a Salt Date Performed: March 1‚ 2011 INTRODUCTION If solid KHC4H4O6 is added to a beaker of water‚ the salt will begin to dissolve. The amount of solid diminishes‚ and the concentrations of K+(aq) and HC4H4O6-(aq) in the solution increase. When no KHC4H4O6 dissolves‚ the concentrations of K+(aq) and HC4H4O6-(aq) will not increase further and any additional KHC4H4O6 added after this point will remain
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In this experiment the initial mass of the sodium carbonate used was 2.69g. In each titration‚ 3 drops of methyl orange was added to the sodium carbonate solution. With this information the titration can begin‚ and the results obtained are shown below: Titration readings Titration Rough 1 2 3 4 5 Initial 0.00 4.30 22.00 21.00 15.00 25.90 Final 4.30 22.00 38.60 37.60 32.60 42.20 Titre (cm3) 4.30 17.70 16.60 16.60 17.60 16.30 Therefore‚ the average titre would be calculated as follows;
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Experiments #7A & 7B 7A. Electrochemical Cells 7B. Formation of a Complex Ion Chem 102 Section 3095 Grace H. Kim Dec. 15‚ 2011 Abstract Two experiments were conducted to figure out the value of the formation constant of tetraamminecopper(II)‚ Kf‚ with different methods and which experimental method produces more accurate result. One was electrochemistry using a Daniel cell and the other one was spectrometry by estimating concentration of complex solution using a calibration
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Experiment 5- Standardization of NaOH and determination of Molarity of an unknown Acid Objectives 1. Preparation and standardization of a 0.1M NaOH solution 2. To learn the technique of titration 3. Determination of the concentration of an unknown diprotic acid. Introduction Titration can be traced to the origins of volumetric analysis‚ which began in the late eighteenth century. Study of analytical chemistry began in France and the first burette was made by Francois Antoine Henri
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! ! ! ! Determination of Gas Constant R through Mg and HCl Reaction ! ! ! ! ! !1 of !9 IB Chemistry SL (Jr) Candidate: Yunha Kim Objective ! Candidates will react Magnesium with Hydrochloric acid and collect Hydrogen. The grams of the produced Hydrogen will be calculated and compared to the theoretical yield of Hydrogen. ! Controlled Variables ! Mass of Magnesium Ribbon This will be kept constant as the objective is to determine the Gas Constant (R). If this
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